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ACTA2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6151064

Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACTA2, Monoclonal Antibody
Full Product Name
ACTA2 (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTSA, ACTVS, GIG46) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4A8-2H3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ACTA2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
42,009 Da
Immunogen
Full length recombinant corresponding to aa1-377 from human ACTA2 (AAH17554.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF
Conjugate
HRP

Product Categories/Family for anti-ACTA2 antibody

Applications Tested/Suitable for anti-ACTA2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ACTA2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ACTA2 antibody

Western Blot detection against Immunogen (67.21kD).
Mouse Anti-ACTA2 Monoclonal Antibody – Cat# MBS6151064

Western Blot (WB) of anti-ACTA2 antibody

ACTA2 monoclonal antibody Western Blot analysis of ACTA2 expression in human omentum, serous carcinoma.
Mouse Anti-ACTA2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6151064

Immunofluorescence (IF) of anti-ACTA2 antibody

Immunofluorescence of monoclonal antibody to ACTA2 on HeLa cell. [antibody concentration 1-10ug/ml]
Mouse Anti-ACTA2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6151064

Testing Data of anti-ACTA2 antibody

Detection limit for recombinant GST tagged ACTA2 is ~1ng/ml as a capture antibody.
Mouse Anti-ACTA2 Monoclonal Antibody, Testing Data – Cat# MBS6151064

Other Notes

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA2 purchase

MBS6151064 is an antibody targeting ACTA2. Also known as ACTA2 (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTSA, ACTVS, GIG46) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens actin, alpha 2, smooth muscle, aorta, mRNA
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
Protein Family
NCBI Protein Information
actin, aortic smooth muscle

NCBI Summary for ACTA2

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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