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CAMKK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6151559

MBS6151559 is an antibody targeting CAMKK1. Also known as CAMKK1 (Calcium/Calmodulin-dependent Protein Kinase Kinase 1, CaM-kinase Kinase 1, CaM-KK 1, CaMKK 1, CaMKK 1, Calcium/Calmodulin-dependent Protein Kinase Kinase alpha, CaM-kinase Kinase alpha, CaM-KK… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CAMKK1, Monoclonal Antibody
Full Product Name
CAMKK1 (Calcium/Calmodulin-dependent Protein Kinase Kinase 1, CaM-kinase Kinase 1, CaM-KK 1, CaMKK 1, CaMKK 1, Calcium/Calmodulin-dependent Protein Kinase Kinase alpha, CaM-kinase Kinase alpha, CaM-KK alpha, CaMKK alpha, CaM-kinase IV Kinase, CAMKKA, DKFZ
Product Gene Name
anti-CAMKK1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1F6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CAMKK1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
57,523 Da
Immunogen
Full length recombinant corresponding to aa1-521 from human CAMKK1 (AAH31647) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEGPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLV
Conjugate
HRP

Product Categories/Family for anti-CAMKK1 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-CAMKK1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-CAMKK1 antibody
IHC-P: 1ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CAMKK1 antibody

Western Blot detection against Immunogen (83.31kD).
Mouse Anti-CAMKK1 Monoclonal Antibody – Cat# MBS6151559

Western Blot (WB) of anti-CAMKK1 antibody

Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-CAMKK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6151559

Western Blot (WB) of anti-CAMKK1 antibody

Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody. Lane 1: CAMKK1 transfected lysate (57.31kD). Lane 2: Non-transfected lysate.
Mouse Anti-CAMKK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6151559

Immunohistochemistry (IHC) of anti-CAMKK1 antibody

Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].
Mouse Anti-CAMKK1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6151559

Testing Data of anti-CAMKK1 antibody

Detection limit for recombinant GST tagged CAMKK1 is ~1ng/ml as a capture antibody.
Mouse Anti-CAMKK1 Monoclonal Antibody, Testing Data – Cat# MBS6151559

Western Blot (WB) of anti-CAMKK1 antibody

CAMKK1 monoclonal antibody, Western Blot analysis of CAMKK1 expression in Jurkat.
Mouse Anti-CAMKK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6151559

Other Notes

Small volumes of anti-CAMKK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CAMKK1. It may not necessarily be applicable to this product.

NCBI GI #
21594835
NCBI GeneID
84254
NCBI Accession #
BC031647
NCBI Related Accession #
AAH31647
UniProt Secondary Accession #
Q9BQH3
UniProt Related Accession #
Q8N5S9
NCBI Official Full Name
Homo sapiens calcium/calmodulin-dependent protein kinase kinase 1, alpha, mRNA
NCBI Official Synonym Full Names
calcium/calmodulin dependent protein kinase kinase 1
NCBI Official Symbol
CAMKK1
NCBI Official Synonym Symbols
CAMKKA
Protein Family
Calcium/calmodulin-dependent protein kinase
NCBI Protein Information
calcium/calmodulin-dependent protein kinase kinase 1

NCBI Summary for CAMKK1

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
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Research Articles on CAMKK1

1. CaMKK1 does not play a pivotal role in the calcium signaling cascade regulating adrenal aldosterone production.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CAMKK1 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKK Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKK Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies  Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
 Post NMDA Receptor Activation Events Pathway antibodies  Post NMDA Receptor Activation Events Pathway Diagram

Diseases associated with anti-CAMKK1 antibody

 Disease Name  Pubmed Publications
 Memory Disorders Antibodies  >1 publications with CAMKK1 and Memory Disorders
 Inflammation Antibodies  >1 publications with CAMKK1 and Inflammation
 Neurobehavioral Manifestations Antibodies  >1 publications with CAMKK1 and Neurobehavioral Manifestations
 Nervous System Diseases Antibodies  >1 publications with CAMKK1 and Nervous System Diseases

Organs/Tissues associated with anti-CAMKK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with CAMKK1 and Brain
 Lung Antibodies  >4 publications with CAMKK1 and Lung
 Nerve Antibodies  >2 publications with CAMKK1 and Nerve
 Embryonic Tissue Antibodies  >1 publications with CAMKK1 and Embryonic Tissue
 Muscle Antibodies  >1 publications with CAMKK1 and Muscle
 Pancreas Antibodies  >1 publications with CAMKK1 and Pancreas
 Heart Antibodies  >1 publications with CAMKK1 and Heart
 Ovary Antibodies  >1 publications with CAMKK1 and Ovary
 Kidney Antibodies  >1 publications with CAMKK1 and Kidney
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