NKCC2 Antibody – Rabbit Polyclonal

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+K+Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.

Unlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and macula densa. NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg. Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting, and volume depletion.

Small volumes of anti-NKCC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS615157 is an antibody targeting NKCC2. Also known as NKCC2 (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2). Reactive in Rat. Host: Rabbit.