Rabbit Anti-SPRY2 Polyclonal Antibody – Cat# MBS615240

Immunohistochemistry of Rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4°C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.

Sprouty 2 (SPRY2) Antibody – Rabbit Polyclonal

Name: SPRY2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS615240
Price: 1140 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS615240

Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling. Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras. Sprouty expression is induced by… More details

$1140 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Sprouty 2 (SPRY2), Polyclonal Antibody

Full Product Name

Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039)

Product Gene Name

anti-SPRY2 antibody

Product Synonym Names

Anti -Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Reacts with human Sprouty-2 protein (38kD). Species Crossreactivity: BLAST analysis was used to suggest reactivity with Sprouty 2 from human, monkey, and orangutan.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

34,688 Da

Chromosome Location

Chromosome: 13; NC_000013.10 (80910111..80915086, complement). Location: 13q31.1

OMIM

602466

3D Structure

ModBase 3D Structure for O43597

Immunogen

Human sprouty-2 peptide.

Product Categories/Family for anti-SPRY2 antibody

Antibodies; Abs to Sprouty

Applications Tested/Suitable for anti-SPRY2 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-SPRY2 antibody

Suitable for use in ELISA, Western Blot and Immunofluorescence.
Dilution: Western Blot: 1:500-1:5000; A 38kD band corresponding to human Sprouty-2 is observed.
Detection occurs using HRP Goat anti-Rabbit IgG and ECL.

Other Notes

Small volumes of anti-SPRY2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPRY2 purchase

MBS615240 is an antibody targeting SPRY2. Also known as Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPRY2. It may not necessarily be applicable to this product.

NCBI GI #

2809400

NCBI GeneID

10253

UniProt Primary Accession #

O43597

UniProt Secondary Accession #

Q5T6Z7

UniProt Related Accession #

O43597; Q7Z783

NCBI Official Full Name

Sprouty 2

NCBI Official Synonym Full Names

sprouty homolog 2 (Drosophila)

NCBI Official Symbol

SPRY2

NCBI Official Synonym Symbols

hSPRY2; MGC23039

UniProt Protein Name

Protein sprouty homolog 2

Protein Family

Protein sprouty

UniProt Gene Name

SPRY2

UniProt Entry Name

SPY2_HUMAN

NCBI Protein Information

protein sprouty homolog 2; spry-2; sprouty 2; OTTHUMP00000018543

NCBI Summary for SPRY2

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq]

UniProt Comments for SPRY2

SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Protein type: Adaptor/scaffold; Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: microtubule cytoskeleton; microtubule; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase inhibitor activity; protein serine/threonine kinase activator activity; protein binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; cell fate commitment; negative regulation of nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; sensory perception of sound; establishment of mitotic spindle orientation; negative regulation of Ras protein signal transduction; negative regulation of apoptosis

Research Articles on SPRY2

1. Spry2 expression is downregulated and miRNA-21 is upregulated in the clinical samples of colon cancer, which correlates with clinical stage of disease. Thus, Spry2 functions as a tumor suppressor in colon cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SPRY2 antibody

Reference Product	PubMed Publications
CBL antibody	>25 publications with SPRY2 and CBL
GRB2 antibody	>11 publications with SPRY2 and GRB2
RAF1 antibody	>2 publications with SPRY2 and RAF1
PTPN11 antibody	>2 publications with SPRY2 and PTPN11

Pathways associated with anti-SPRY2 antibody

Products by Pathway	Pathway Diagram
EGFR Downregulation Pathway antibodies	EGFR Downregulation Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
FGF Signaling Pathway antibodies	FGF Signaling Pathway Diagram
Internalization Of ErbB1 Pathway antibodies	Internalization Of ErbB1 Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Signaling By EGFR Pathway antibodies	Signaling By EGFR Pathway Diagram
Signaling Events Mediated By PTP1B Pathway antibodies	Signaling Events Mediated By PTP1B Pathway Diagram

Diseases associated with anti-SPRY2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>45 publications with SPRY2 and Neoplasms
Carcinoma Antibodies	>17 publications with SPRY2 and Carcinoma
Adenocarcinoma Antibodies	>13 publications with SPRY2 and Adenocarcinoma
Melanoma Antibodies	>7 publications with SPRY2 and Melanoma
Cell Transformation, Neoplastic Antibodies	>7 publications with SPRY2 and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>6 publications with SPRY2 and Liver Diseases
Lung Neoplasms Antibodies	>6 publications with SPRY2 and Lung Neoplasms
Liver Neoplasms Antibodies	>6 publications with SPRY2 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>6 publications with SPRY2 and Carcinoma, Hepatocellular
Craniofacial Abnormalities Antibodies	>6 publications with SPRY2 and Craniofacial Abnormalities

Organs/Tissues associated with anti-SPRY2 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>57 publications with SPRY2 and Nerve
Embryonic Tissue Antibodies	>30 publications with SPRY2 and Embryonic Tissue
Lung Antibodies	>27 publications with SPRY2 and Lung
Brain Antibodies	>22 publications with SPRY2 and Brain
Muscle Antibodies	>12 publications with SPRY2 and Muscle
Eye Antibodies	>7 publications with SPRY2 and Eye
Liver Antibodies	>6 publications with SPRY2 and Liver
Bone Antibodies	>5 publications with SPRY2 and Bone
Kidney Antibodies	>5 publications with SPRY2 and Kidney
Prostate Antibodies	>5 publications with SPRY2 and Prostate