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EZR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6152417

MBS6152417 is an antibody targeting EZR. Also known as EZR (Ezrin, Cytovillin, Villin-2, p81, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EZR, Monoclonal Antibody
Full Product Name
EZR (Ezrin, Cytovillin, Villin-2, p81, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584) (HRP)
Product Gene Name
anti-EZR antibody
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1E11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human EZR.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
69,413 Da
OMIM
123900
3D Structure
ModBase 3D Structure for P15311
Immunogen
Full length recombinant corresponding to aa1-586 from human EZR (AAH13903) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL
Conjugate
HRP

Product Categories/Family for anti-EZR antibody

Antibodies; Actin Related Proteins
Applications Tested/Suitable for anti-EZR antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-EZR antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EZR antibody

VIL2 monoclonal antibody. Western Blot analysis of VIL2 expression in HepG2.
Mouse Anti-EZR Monoclonal Antibody – Cat# MBS6152417

Immunohistochemistry (IHC) of anti-EZR antibody

Immunoperoxidase of monoclonal antibody to EZR on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml]
Mouse Anti-EZR Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6152417

Testing Data of anti-EZR antibody

Detection limit for recombinant GST tagged EZR is 3ng/ml as a capture antibody.
Mouse Anti-EZR Monoclonal Antibody, Testing Data – Cat# MBS6152417

Testing Data of anti-EZR antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between FADD and EZR. HeLa cells were stained with FADD rabbit purified polyclonal 1:1200 and EZR mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Mouse Anti-EZR Monoclonal Antibody, Testing Data – Cat# MBS6152417

Other Notes

Small volumes of anti-EZR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EZR. It may not necessarily be applicable to this product.

NCBI GI #
33869413
NCBI GeneID
7430
NCBI Accession #
BC013903
NCBI Related Accession #
AAH13903
UniProt Primary Accession #
P15311
UniProt Secondary Accession #
P23714; Q4VX75; Q96CU8; Q9NSJ4; E1P5A8
UniProt Related Accession #
P15311
NCBI Official Full Name
Homo sapiens ezrin, mRNA
NCBI Official Synonym Full Names
ezrin
NCBI Official Symbol
EZR
NCBI Official Synonym Symbols
CVL; CVIL; VIL2; HEL-S-105
UniProt Protein Name
Ezrin
UniProt Synonym Protein Names
Cytovillin; Villin-2; p81
Protein Family
Ezrin
UniProt Gene Name
EZR
UniProt Synonym Gene Names
VIL2
UniProt Entry Name
EZRI_HUMAN
NCBI Protein Information
ezrin

NCBI Summary for EZR

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for EZR

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; focal adhesion; microvillus; basolateral plasma membrane; T-tubule; actin filament; cytosol; actin cytoskeleton; lipid raft; ruffle; extrinsic to membrane; membrane; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; nucleolus; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: microvillus biogenesis; axon guidance; actin filament bundle formation; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity
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Research Articles on EZR

1. We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-EZR antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FAS (CD95) Signaling Pathway antibodies  FAS (CD95) Signaling Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 L1CAM Interactions Pathway antibodies  L1CAM Interactions Pathway Diagram
 LKB1 Signaling Events Pathway antibodies  LKB1 Signaling Events Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-EZR antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with EZR and Neoplasms
 Carcinoma Antibodies  >2 publications with EZR and Carcinoma
 Disease Models, Animal Antibodies  >1 publications with EZR and Disease Models, Animal
 Adenocarcinoma Antibodies  >1 publications with EZR and Adenocarcinoma
 Breast Neoplasms Antibodies  >1 publications with EZR and Breast Neoplasms
 Inflammation Antibodies  >1 publications with EZR and Inflammation

Organs/Tissues associated with anti-EZR antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >2 publications with EZR and Bone
 Lung Antibodies  >2 publications with EZR and Lung
 Blood Antibodies  >1 publications with EZR and Blood
 Bone Marrow Antibodies  >1 publications with EZR and Bone Marrow
 Lymph Antibodies  >1 publications with EZR and Lymph
 Thyroid Antibodies  >1 publications with EZR and Thyroid
 Connective Tissue Antibodies  >1 publications with EZR and Connective Tissue
 Lymph Node Antibodies  >1 publications with EZR and Lymph Node
 Eye Antibodies  >1 publications with EZR and Eye
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