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GAD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6152612

Glutamic acid decarboxylase (GAD) catalyzes the conversion of L-glutamic acid to the inhibitory eurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, re-encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
GAD1, Monoclonal Antibody
Full Product Name
GAD1 (Glutamate Decarboxylase 1, 67kD Glutamic Acid Decarboxylase, GAD-67, Glutamate Decarboxylase 67kD Isoform, GAD, GAD67) (HRP)
Product Gene Name
anti-GAD1 antibody
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
5E11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human GAD1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,440 Da
Immunogen
Full length recombinant corresponding to aa1-594 from human GAD1 (AAH37780) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEQSRYSIKKAGAALGFGTDNVILIKCNERGEIIPADFEAKILEAKQKGYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL
Conjugate
HRP

Product Categories/Family for anti-GAD1 antibody

Antibodies; Enzymes; Decarboxylase
Applications Tested/Suitable for anti-GAD1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-GAD1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GAD1 antibody

Western Blot detection against Immunogen (91.08kD).
Mouse Anti-GAD1 Monoclonal Antibody – Cat# MBS6152612

Western Blot (WB) of anti-GAD1 antibody

Western Blot analysis of GAD1 expression in transfected 293T cell line by GAD1 monoclonal antibody. Lane 1: GAD1 transfected lysate (66.9kD). Lane 2: Non-transfected lysate.
Mouse Anti-GAD1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6152612

Other Notes

Small volumes of anti-GAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD1 purchase

MBS6152612 is an antibody targeting GAD1. Also known as GAD1 (Glutamate Decarboxylase 1, 67kD Glutamic Acid Decarboxylase, GAD-67, Glutamate Decarboxylase 67kD Isoform, GAD, GAD67) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.

NCBI GI #
34192798
NCBI GeneID
2571
NCBI Accession #
BC037780
NCBI Related Accession #
AAH37780
UniProt Secondary Accession #
Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
UniProt Related Accession #
Q99259
NCBI Official Full Name
Homo sapiens glutamate decarboxylase 1 (brain, 67kDa), mRNA
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
Protein Family
Glutamate decarboxylase
NCBI Protein Information
glutamate decarboxylase 1

NCBI Summary for GAD1

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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Research Articles on GAD1

1. This study showed that in female groups, the expression of GABRA5 was generally higher in schizophrenia cases compared to the control.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GAD1 antibody

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with anti-GAD1 antibody

 Disease Name  Pubmed Publications
 Bipolar Disorder Antibodies  >13 publications with GAD1 and Bipolar Disorder
 Disease Models, Animal Antibodies  >11 publications with GAD1 and Disease Models, Animal
 Seizures Antibodies  >9 publications with GAD1 and Seizures
 Autistic Disorder Antibodies  >5 publications with GAD1 and Autistic Disorder
 Cognition Disorders Antibodies  >4 publications with GAD1 and Cognition Disorders
 Neurobehavioral Manifestations Antibodies  >3 publications with GAD1 and Neurobehavioral Manifestations
 Cardiovascular Diseases Antibodies  >2 publications with GAD1 and Cardiovascular Diseases
 Nerve Degeneration Antibodies  >1 publications with GAD1 and Nerve Degeneration
 Necrosis Antibodies  >1 publications with GAD1 and Necrosis
 Learning Disorders Antibodies  >1 publications with GAD1 and Learning Disorders

Organs/Tissues associated with anti-GAD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with GAD1 and Muscle
 Kidney Antibodies  >2 publications with GAD1 and Kidney
 Embryonic Tissue Antibodies  >1 publications with GAD1 and Embryonic Tissue
 Intestine Antibodies  >1 publications with GAD1 and Intestine
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