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KLF15 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6153218

KLF15 is a transcriptional activator. Binds to the GA element of the CLCNKA promoter. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLF15, Monoclonal Antibody
Full Product Name
KLF15 (Krueppel-like Factor 15, Kidney-enriched Krueppel-like Factor, KKLF, DKFZp779M1320) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1F3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human KLF15.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,992 Da
OMIM
606465
3D Structure
ModBase 3D Structure for Q9UIH9
Immunogen
Partial recombinant corresponding to aa1-79 from human KLF15 (NP_054798) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MVDHLLPVDENFSSPKCPVGYLGDRLVGRRAYHMLPSPVSEDDSDASSPCSCSSPDSQALCSCYGGGLGTESQDSILD*
Conjugate
HRP
Applications Tested/Suitable for anti-KLF15 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-KLF15 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KLF15 antibody

Western Blot detection against Immunogen (34.69kD).
Mouse Anti-KLF15 Monoclonal Antibody – Cat# MBS6153218

Western Blot (WB) of anti-KLF15 antibody

Western Blot analysis of KLF15 expression in transfected 293T cell line by KLF15 monoclonal antibody. Lane 1: KLF15 transfected lysate (44kD). Lane 2: Non-transfected lysate.
Mouse Anti-KLF15 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153218

Immunoprecipitation (IP) of anti-KLF15 antibody

Immunoprecipitation of KLF15 transfected lysate using KLF15 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with KLF15 rabbit polyclonal antibody.
Mouse Anti-KLF15 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6153218

Testing Data of anti-KLF15 antibody

Detection limit for recombinant GST tagged KLF15 is 1ng/ml as a capture antibody.
Mouse Anti-KLF15 Monoclonal Antibody, Testing Data – Cat# MBS6153218

Other Notes

Small volumes of anti-KLF15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF15 purchase

MBS6153218 is an antibody targeting KLF15. Also known as KLF15 (Krueppel-like Factor 15, Kidney-enriched Krueppel-like Factor, KKLF, DKFZp779M1320) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Krueppel-like factor 15
NCBI Official Synonym Full Names
Kruppel-like factor 15
NCBI Official Symbol
KLF15
NCBI Official Synonym Symbols
KKLF
UniProt Protein Name
Krueppel-like factor 15
UniProt Synonym Protein Names
Kidney-enriched krueppel-like factor
Protein Family
UniProt Gene Name
KLF15
UniProt Synonym Gene Names
KKLF
UniProt Entry Name
KLF15_HUMAN
NCBI Protein Information
Krueppel-like factor 15; kidney-enriched Kruppel-like factor; kidney-enriched krueppel-like factor

UniProt Comments for KLF15

KLF15: Transcriptional activator. Binds to the GA element of the CLCNKA promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding

Biological Process: cardiac muscle hypertrophy; glial cell differentiation; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KLF15 antibody

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