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NFKB1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6153775

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50kD protein is a DNA binding subunit of the… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NFKB1, Monoclonal Antibody
Full Product Name
NFKB1 (Nuclear Factor NF-kappa-B p105 Subunit, DNA-binding Factor KBF1, EBP-1, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 1, Nuclear Factor NF-kappa-B p50 Subunit, DKFZp686C01211, MGC54151) (HRP)
Product Gene Name
anti-NFKB1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2E6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NFKB1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
85,520 Da
Immunogen
Partial recombinant corresponding to aa860-969 from human NFKB1 (AAH51765) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
Conjugate
HRP

Product Categories/Family for anti-NFKB1 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-NFKB1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NFKB1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFKB1 antibody

Western Blot detection against Immunogen (37.73kD).
Mouse Anti-NFKB1 Monoclonal Antibody – Cat# MBS6153775

Western Blot (WB) of anti-NFKB1 antibody

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody. Lane 1: NFKB1 transfected lysate (105.4kD). Lane 2: Non-transfected lysate.
Mouse Anti-NFKB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153775

Immunofluorescence (IF) of anti-NFKB1 antibody

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-NFKB1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6153775

Western Blot (WB) of anti-NFKB1 antibody

Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFKB1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-NFKB1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153775

Testing Data of anti-NFKB1 antibody

Proximity Ligation Analysis of protein-protein interactions between IKBKB and NFKB1. HeLa cells were stained with IKBKB rabbit purified polyclonal 1:1200 and NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-NFKB1 Monoclonal Antibody, Testing Data – Cat# MBS6153775

Other Notes

Small volumes of anti-NFKB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFKB1 purchase

MBS6153775 is an antibody targeting NFKB1. Also known as NFKB1 (Nuclear Factor NF-kappa-B p105 Subunit, DNA-binding Factor KBF1, EBP-1, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 1, Nuclear Factor NF-kappa-B p50 Subunit, DKFZp686C01211, MGC54151) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFKB1. It may not necessarily be applicable to this product.

NCBI GI #
30354569
NCBI GeneID
4790
NCBI Accession #
BC051765
NCBI Related Accession #
AAH51765
UniProt Secondary Accession #
Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
UniProt Related Accession #
P19838
NCBI Official Full Name
Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, mRNA
NCBI Official Synonym Full Names
nuclear factor kappa B subunit 1
NCBI Official Symbol
NFKB1
NCBI Official Synonym Symbols
p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Protein Family
Nuclear factor
NCBI Protein Information
nuclear factor NF-kappa-B p105 subunit

NCBI Summary for NFKB1

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
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Research Articles on NFKB1

1. JMJD3 up-regulation and NF-kappaB activation occur in the region of the wound edge during keratinocyte wound healing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NFKB1 antibody

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alternative NF-kappaB Pathway antibodies  Alternative NF-kappaB Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram

Diseases associated with anti-NFKB1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >129 publications with NFKB1 and Neoplasms
 Inflammation Antibodies  >126 publications with NFKB1 and Inflammation
 Necrosis Antibodies  >92 publications with NFKB1 and Necrosis
 Cardiovascular Diseases Antibodies  >45 publications with NFKB1 and Cardiovascular Diseases
 Nervous System Diseases Antibodies  >19 publications with NFKB1 and Nervous System Diseases
 Liver Diseases Antibodies  >18 publications with NFKB1 and Liver Diseases
 Brain Diseases Antibodies  >14 publications with NFKB1 and Brain Diseases
 Heart Diseases Antibodies  >12 publications with NFKB1 and Heart Diseases
 Drug Toxicity Antibodies  >12 publications with NFKB1 and Drug Toxicity
 Cell Transformation, Neoplastic Antibodies  >10 publications with NFKB1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-NFKB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >139 publications with NFKB1 and Blood
 Bone Antibodies  >42 publications with NFKB1 and Bone
 Vascular Antibodies  >38 publications with NFKB1 and Vascular
 Liver Antibodies  >35 publications with NFKB1 and Liver
 Bone Marrow Antibodies  >30 publications with NFKB1 and Bone Marrow
 Lung Antibodies  >26 publications with NFKB1 and Lung
 Brain Antibodies  >23 publications with NFKB1 and Brain
 Kidney Antibodies  >19 publications with NFKB1 and Kidney
 Muscle Antibodies  >17 publications with NFKB1 and Muscle
 Heart Antibodies  >15 publications with NFKB1 and Heart
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