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NFYB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6153779

Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFYB, Monoclonal Antibody
Full Product Name
NFYB (Nuclear Transcription Factor Y Subunit beta, CAAT Box DNA-binding Protein Subunit B, Nuclear Transcription Factor Y Subunit B, NF-YB, HAP3) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6H6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human NFYB.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-208 from human NFYB (AAH05317) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Conjugate
HRP
Applications Tested/Suitable for anti-NFYB antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-NFYB antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFYB antibody

Western Blot detection against Immunogen (48.51kD).
Mouse Anti-NFYB Monoclonal Antibody – Cat# MBS6153779

Western Blot (WB) of anti-NFYB antibody

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody Lane 1: NFYB transfected lysate (22.8kD). Lane 2: Non-transfected lysate.
Mouse Anti-NFYB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153779

Testing Data of anti-NFYB antibody

Detection limit for recombinant GST tagged NFYB is ~0.1ng/ml as a capture antibody.
Mouse Anti-NFYB Monoclonal Antibody, Testing Data – Cat# MBS6153779

Western Blot (WB) of anti-NFYB antibody

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-NFYB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153779

Other Notes

Small volumes of anti-NFYB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFYB purchase

MBS6153779 is an antibody targeting NFYB. Also known as NFYB (Nuclear Transcription Factor Y Subunit beta, CAAT Box DNA-binding Protein Subunit B, Nuclear Transcription Factor Y Subunit B, NF-YB, HAP3) (HRP). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFYB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens nuclear transcription factor Y, beta, mRNA
NCBI Official Synonym Full Names
nuclear transcription factor Y subunit beta
NCBI Official Symbol
NFYB
NCBI Official Synonym Symbols
HAP3; CBF-A; CBF-B; NF-YB
NCBI Protein Information
nuclear transcription factor Y subunit beta

NCBI Summary for NFYB

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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