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ORAI1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS615386

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels (1 for review). ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this… More details

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Product Overview

Online Datasheet in PDF
Product Name
ORAI1, Polyclonal Antibody
Full Product Name
ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)
Product Gene Name
anti-ORAI1 antibody
Product Synonym Names
Anti -ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ORAI1. Predicted to have no crossreactivity to ORAI2 or ORAI3. Species Crossreactivity: Mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
32,668 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (122064455..122079946). Location: 12q24.31
OMIM
610277
3D Structure
ModBase 3D Structure for Q96D31
Immunogen
Synthetic peptide correspoding to 18aa from near the amino terminus of human ORAI1 (GenBank Q96D31).
Positive Control
WB: Human ovary tissue lysate
IHC: Human spleen tissue

Product Categories/Family for anti-ORAI1 antibody

Antibodies; Abs to Membrane Proteins
Applications Tested/Suitable for anti-ORAI1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ORAI1 antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-1ug/ml
Immunohistochemistry: 10ug/ml

Western Blot (WB) of anti-ORAI1 antibody

Western blot analysis of ORAI1 in human ovary tissue lysate with MBS615386 at 1ug/ml in the (A) absence or (B) presence of MBS615386-01 blocking peptide.
Rabbit Anti-ORAI1 Polyclonal Antibody – Cat# MBS615386

Immunohistochemistry (IHC) of anti-ORAI1 antibody

Immunohistochemistry of ORAI1 in human spleen tissue with MBS615386 at 10ug/ml.
Rabbit Anti-ORAI1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS615386

Other Notes

Small volumes of anti-ORAI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ORAI1 purchase

MBS615386 is an antibody targeting ORAI1. Also known as ORAI1 (Transmembrane Protein 142A, TMEM142A, Calcium Release-Activated Calcium Channel Protein 1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ORAI1. It may not necessarily be applicable to this product.

NCBI GI #
15426546
NCBI GeneID
84876
UniProt Primary Accession #
Q96D31
UniProt Secondary Accession #
Q3MHV3; Q6DHX2; Q96BP7; Q96K71
UniProt Related Accession #
Q96D31
NCBI Official Full Name
ORAI1 protein
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol
ORAI1
NCBI Official Synonym Symbols
ORAT1; CRACM1; FLJ14466; TMEM142A
UniProt Protein Name
Calcium release-activated calcium channel protein 1
UniProt Synonym Protein Names
Protein orai-1; Transmembrane protein 142A
UniProt Gene Name
ORAI1
UniProt Synonym Gene Names
CRACM1; TMEM142A
UniProt Entry Name
CRCM1_HUMAN
NCBI Protein Information
calcium release-activated calcium channel protein 1; protein orai-1; OTTHUMP00000240038; transmembrane protein 142A; calcium release-activated calcium modulator 1

NCBI Summary for ORAI1

CRACM1 is a plasma membrane protein essential for store-operated calcium entry (Vig et al., 2006 [PubMed 16645049]).[supplied by OMIM]

UniProt Comments for ORAI1

ORAI1: Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Interacts with STIM1 and STIM2. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and STIM1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Belongs to the Orai family.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: protein complex; membrane; integral to plasma membrane

Molecular Function: calmodulin binding; identical protein binding; protein binding; store-operated calcium channel activity

Biological Process: immune system process; regulation of calcium ion transport; positive regulation of calcium ion transport; blood coagulation

Disease: Myopathy, Tubular Aggregate, 2; Immunodeficiency 9
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Research Articles on ORAI1

1. Expression of exogenous wild-type Orai1 rescues vascular endothelial growth factor (VEGF)-induced endothelial tube formation in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ORAI1 antibody

 Reference Product  PubMed Publications
 TRPC3 antibody  >21 publications with ORAI1 and TRPC3
 TRPC6 antibody  >18 publications with ORAI1 and TRPC6

Pathways associated with anti-ORAI1 antibody

 Products by Pathway  Pathway Diagram
 Elevation Of Cytosolic Ca2+ Levels Pathway antibodies  Elevation Of Cytosolic Ca2+ Levels Pathway Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Platelet Calcium Homeostasis Pathway antibodies  Platelet Calcium Homeostasis Pathway Diagram
 Platelet Homeostasis Pathway antibodies  Platelet Homeostasis Pathway Diagram
 TCR Signaling In Naive CD4+ T Cells Pathway antibodies  TCR Signaling In Naive CD4+ T Cells Pathway Diagram
 TCR Signaling In Naive CD8+ T Cells Pathway antibodies  TCR Signaling In Naive CD8+ T Cells Pathway Diagram

Diseases associated with anti-ORAI1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with ORAI1 and Inflammation
 Kidney Diseases Antibodies  >5 publications with ORAI1 and Kidney Diseases
 Myocardial Ischemia Antibodies  >4 publications with ORAI1 and Myocardial Ischemia
 Congenital Abnormalities Antibodies  >4 publications with ORAI1 and Congenital Abnormalities
 Breast Neoplasms Antibodies  >4 publications with ORAI1 and Breast Neoplasms
 Hypertension Antibodies  >4 publications with ORAI1 and Hypertension
 Drug Toxicity Antibodies  >3 publications with ORAI1 and Drug Toxicity
 Carcinoma Antibodies  >2 publications with ORAI1 and Carcinoma
 Hyperalgesia Antibodies  >1 publications with ORAI1 and Hyperalgesia
 Neurodegenerative Diseases Antibodies  >1 publications with ORAI1 and Neurodegenerative Diseases

Organs/Tissues associated with anti-ORAI1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >74 publications with ORAI1 and Blood
 Kidney Antibodies  >22 publications with ORAI1 and Kidney
 Heart Antibodies  >16 publications with ORAI1 and Heart
 Lung Antibodies  >11 publications with ORAI1 and Lung
 Brain Antibodies  >8 publications with ORAI1 and Brain
 Bone Antibodies  >5 publications with ORAI1 and Bone
 Salivary Gland Antibodies  >5 publications with ORAI1 and Salivary Gland
 Eye Antibodies  >4 publications with ORAI1 and Eye
 Embryonic Tissue Antibodies  >4 publications with ORAI1 and Embryonic Tissue
 Intestine Antibodies  >3 publications with ORAI1 and Intestine
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