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Oncofetal Antigen 5t4 (TPBG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6153921

Oncofetal antigen 5T4, also TPBG and trophoblast glycoprotein, is a 72kD glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and… More details

$1140 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Oncofetal Antigen 5T4 (TPBG), Monoclonal Antibody
Full Product Name
Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1B6
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TPBG. Species Crossreactivity: rat.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
190920
3D Structure
ModBase 3D Structure for Q13641
Immunogen
Partial recombinant corresponding to aa219-328 from human TPBG (NP_006661) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEK
Conjugate
HRP
Applications Tested/Suitable for anti-TPBG antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-TPBG antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TPBG antibody

TPBG monoclonal antibody. Western Blot analysis of TPBG expression in HeLa.
Mouse Anti-TPBG Monoclonal Antibody – Cat# MBS6153921

Western Blot (WB) of anti-TPBG antibody

TPBG monoclonal antibody. Western Blot analysis of TPBG expression in PC-12.
Mouse Anti-TPBG Monoclonal Antibody, Western Blot (WB) – Cat# MBS6153921

Testing Data of anti-TPBG antibody

Detection limit for recombinant GST tagged TPBG is ~1ng/ml as a capture antibody.
Mouse Anti-TPBG Monoclonal Antibody, Testing Data – Cat# MBS6153921

Other Notes

Small volumes of anti-TPBG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPBG purchase

MBS6153921 is an antibody targeting TPBG. Also known as Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1) (HRP). Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TPBG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
trophoblast glycoprotein
UniProt Protein Name
Trophoblast glycoprotein
UniProt Synonym Protein Names
5T4 oncofetal antigen; 5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; M6P1
UniProt Gene Name
TPBG
UniProt Synonym Gene Names
5T4; 5T4 oncotrophoblast glycoprotein
UniProt Entry Name
TPBG_HUMAN

UniProt Comments for TPBG

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Tissue specificity: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Miscellaneous: Antigen 5T4 is overexpressed by a wide spectrum of cancers, including colorectal, ovarian and gastric, but with a limited normal tissue expression. Could be used for tumor immunotherapy.

Sequence similarities: Contains 6 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.Contains 1 LRRNT domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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