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PIK3R1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6154170

PI3KR1 binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the P110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PIK3R1, Monoclonal Antibody
Full Product Name
PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (HRP)
Product Gene Name
anti-PIK3R1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3A10
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PIK3R1. Species Crossreactivity: mouse and rat.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
42,838 Da
Immunogen
Full length recombinant corresponding to aa1-454 from human PIK3R1 (AAH30815) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MYNTVWNMEDLDLEYAKTDINCGTDLMFYIEMDPPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHKYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR
Conjugate
HRP

Product Categories/Family for anti-PIK3R1 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-PIK3R1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PIK3R1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PIK3R1 antibody

Western Blot detection against Immunogen (75.68kD).
Mouse Anti-PIK3R1 Monoclonal Antibody – Cat# MBS6154170

Western Blot (WB) of anti-PIK3R1 antibody

PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.
Mouse Anti-PIK3R1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6154170

Western Blot (WB) of anti-PIK3R1 antibody

PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.
Mouse Anti-PIK3R1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6154170

Testing Data of anti-PIK3R1 antibody

Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-PIK3R1 Monoclonal Antibody, Testing Data – Cat# MBS6154170

Testing Data of anti-PIK3R1 antibody

Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue)
Mouse Anti-PIK3R1 Monoclonal Antibody, Testing Data – Cat# MBS6154170

Testing Data of anti-PIK3R1 antibody

Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-PIK3R1 Monoclonal Antibody, Testing Data – Cat# MBS6154170

Testing Data of anti-PIK3R1 antibody

Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-PIK3R1 Monoclonal Antibody, Testing Data – Cat# MBS6154170

Other Notes

Small volumes of anti-PIK3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIK3R1 purchase

MBS6154170 is an antibody targeting PIK3R1. Also known as PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (HRP). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3R1. It may not necessarily be applicable to this product.

NCBI GI #
21410089
NCBI GeneID
5295
NCBI Accession #
BC030815
NCBI Related Accession #
AAH30815
UniProt Secondary Accession #
Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
UniProt Related Accession #
P27986
NCBI Official Full Name
Homo sapiens phosphoinositide-3-kinase, regulatory subunit 1 (alpha), mRNA
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 1
NCBI Official Symbol
PIK3R1
NCBI Official Synonym Symbols
p85; AGM7; GRB1; IMD36; p85-ALPHA
Protein Family
Phosphatidylinositol 3-kinase regulatory
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit alpha

NCBI Summary for PIK3R1

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
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Research Articles on PIK3R1

1. RAC1/RAC2 and SFK are proximal and essential for phosphatidylinositol 3-kinase (PI3K) activation in NK cell-mediated direct cytotoxicity against Cryptococcus neoformans.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PIK3R1 antibody

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram

Diseases associated with anti-PIK3R1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >48 publications with PIK3R1 and Neoplasms
 Insulin Resistance Antibodies  >14 publications with PIK3R1 and Insulin Resistance
 Carcinoma Antibodies  >12 publications with PIK3R1 and Carcinoma
 Brain Diseases Antibodies  >8 publications with PIK3R1 and Brain Diseases
 Nervous System Diseases Antibodies  >8 publications with PIK3R1 and Nervous System Diseases
 Adenocarcinoma Antibodies  >7 publications with PIK3R1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >5 publications with PIK3R1 and Breast Neoplasms
 Inflammation Antibodies  >5 publications with PIK3R1 and Inflammation
 Cell Transformation, Neoplastic Antibodies  >5 publications with PIK3R1 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >4 publications with PIK3R1 and Neoplasms, Experimental

Organs/Tissues associated with anti-PIK3R1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with PIK3R1 and Brain
 Connective Tissue Antibodies  >7 publications with PIK3R1 and Connective Tissue
 Bone Marrow Antibodies  >3 publications with PIK3R1 and Bone Marrow
 Heart Antibodies  >2 publications with PIK3R1 and Heart
 Testis Antibodies  >1 publications with PIK3R1 and Testis
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