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RAB1B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6154464

MBS6154464 is an antibody targeting RAB1B. Also known as RAB1B (Ras-related protein Rab-1B) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAB1B, Monoclonal Antibody
Full Product Name
RAB1B (Ras-related protein Rab-1B) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1B2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human RAB1B.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
24.3 kDa (221aa), confirmed by MALDI-TOF
OMIM
612565
3D Structure
ModBase 3D Structure for Q9H0U4
Immunogen
Partial recombinant corresponding to aa91-202 from human RAB1B (NP_112243) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC
Conjugate
HRP

Product Categories/Family for anti-RAB1B antibody

Applications Tested/Suitable for anti-RAB1B antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-RAB1B antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RAB1B antibody

Western Blot detection against Immunogen (38.32kD).
Mouse Anti-RAB1B Monoclonal Antibody – Cat# MBS6154464

Western Blot (WB) of anti-RAB1B antibody

RAB1B monoclonal antibody. Western Blot analysis of RAB1B expression in A-431.
Mouse Anti-RAB1B Monoclonal Antibody, Western Blot (WB) – Cat# MBS6154464

Western Blot (WB) of anti-RAB1B antibody

Western Blot analysis of RAB1B expression in transfected 293T cell line by RAB1B monoclonal antibody. Lane 1: RAB1B transfected lysate (22.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-RAB1B Monoclonal Antibody, Western Blot (WB) – Cat# MBS6154464

Immunohistochemistry (IHC) of anti-RAB1B antibody

Immunoperoxidase of monoclonal antibody to RAB1B on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3ug/ml].
Mouse Anti-RAB1B Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6154464

Testing Data of anti-RAB1B antibody

Detection limit for recombinant GST tagged RAB1B is 0.03ng/ml as a capture antibody.
Mouse Anti-RAB1B Monoclonal Antibody, Testing Data – Cat# MBS6154464

Other Notes

Small volumes of anti-RAB1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAB1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-related protein Rab-1B
NCBI Official Synonym Full Names
RAB1B, member RAS oncogene family
NCBI Official Symbol
RAB1B
UniProt Protein Name
Ras-related protein Rab-1B
Protein Family
UniProt Gene Name
RAB1B
UniProt Entry Name
RAB1B_HUMAN
NCBI Protein Information
ras-related protein Rab-1B

NCBI Summary for RAB1B

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009]
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UniProt Comments for RAB1B

RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RAB1B antibody

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