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SSX2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155208

MBS6155208 is an antibody targeting SSX2. Also known as SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40, MGC119055, MGC15364, MGC3884) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SSX2, Monoclonal Antibody
Full Product Name
SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40, MGC119055, MGC15364, MGC3884) (HRP)
Product Gene Name
anti-SSX2 antibody
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1A4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SSX2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,173 Da
Immunogen
Full-length recombinant corresponding to aa1-224 from human SSX2 (AAH02818) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW*
Conjugate
HRP

Product Categories/Family for anti-SSX2 antibody

Antibodies; Cancer Markers
Applications Tested/Suitable for anti-SSX2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-SSX2 antibody
IHC-P: 1.5ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SSX2 antibody

Western Blot detection against Immunogen (50.64kD).
Mouse Anti-SSX2 Monoclonal Antibody – Cat# MBS6155208

Western Blot (WB) of anti-SSX2 antibody

Western Blot analysis of SSX2 expression in transfected 293T cell line by SSX2 monoclonal antibody. Lane 1: SSX2 transfected lysate (25.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-SSX2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155208

Immunohistochemistry (IHC) of anti-SSX2 antibody

Immunoperoxidase of monoclonal antibody to SSX2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5ug/ml].
Mouse Anti-SSX2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6155208

Testing Data of anti-SSX2 antibody

Detection limit for recombinant GST tagged SSX2 is ~0.03ng/ml as a capture antibody.
Mouse Anti-SSX2 Monoclonal Antibody, Testing Data – Cat# MBS6155208

Product References and Citations for anti-SSX2 antibody

1. Expression and Immunotherapeutic Targeting of the SSX Family of Cancer-Testis Antigens in Prostate Cancer. Smith HA, Cronk RJ, Lang JM, McNeel DG.Cancer Res. 2011 Sep 7.

Other Notes

Small volumes of anti-SSX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SSX2. It may not necessarily be applicable to this product.

NCBI GI #
33877218
NCBI GeneID
6757
NCBI Accession #
BC002818
NCBI Related Accession #
AAH02818
UniProt Secondary Accession #
Q16404; Q5JS26; Q96IP7; Q9BU88
UniProt Related Accession #
Q16385
NCBI Official Full Name
Homo sapiens synovial sarcoma, X breakpoint 2, mRNA
NCBI Official Synonym Full Names
SSX family member 2
NCBI Official Symbol
SSX2
NCBI Official Synonym Symbols
SSX; HD21; CT5.2; CT5.2A; HOM-MEL-40
Protein Family
Protein
NCBI Protein Information
protein SSX2

NCBI Summary for SSX2

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 727837, located about 45 kb downstream in the opposite orientation on chromosome X. [provided by RefSeq, Jul 2013]
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Research Articles on SSX2

1. results reveal two important phenotypes of ectopic SSX2 expression that may drive/support tumorigenesis: First, immediate induction of genomic instability, and second, long-term support of tumor cell growth.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SSX2 antibody

 Products by Pathway  Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-SSX2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >180 publications with SSX2 and Neoplasms
 Sarcoma, Synovial Antibodies  >171 publications with SSX2 and Sarcoma, Synovial
 Lung Neoplasms Antibodies  >22 publications with SSX2 and Lung Neoplasms
 Carcinoma Antibodies  >14 publications with SSX2 and Carcinoma
 Breast Neoplasms Antibodies  >11 publications with SSX2 and Breast Neoplasms
 Kidney Neoplasms Antibodies  >10 publications with SSX2 and Kidney Neoplasms
 Head and Neck Neoplasms Antibodies  >10 publications with SSX2 and Head and Neck Neoplasms
 Adenocarcinoma Antibodies  >8 publications with SSX2 and Adenocarcinoma
 Skin Neoplasms Antibodies  >7 publications with SSX2 and Skin Neoplasms
 Leukemia Antibodies  >5 publications with SSX2 and Leukemia

Organs/Tissues associated with anti-SSX2 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >48 publications with SSX2 and Testis
 Skin Antibodies  >8 publications with SSX2 and Skin
 Bone Marrow Antibodies  >5 publications with SSX2 and Bone Marrow
 Liver Antibodies  >2 publications with SSX2 and Liver
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