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STAG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155219

MBS6155219 is an antibody targeting STAG1. Also known as STAG1 (Stromal Antigen 1, SA1, Cohesin Subunit SA-1, SCC3 Homolog 1, SCC3A) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
STAG1, Monoclonal Antibody
Full Product Name
STAG1 (Stromal Antigen 1, SA1, Cohesin Subunit SA-1, SCC3 Homolog 1, SCC3A) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2E9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human STAG1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
140,090 Da
Immunogen
Partial recombinant corresponding to aa1112-1221 from STAG1 (AAH64699) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
LPAPQLTSTVLRENSRPMGDQIQEPESEHGSEPDFLHNRGLMEEDAEPIFEDVMMSSRSQLEDMNEEFEDTMVIDLPPSRNRRERAELRPDFFDSAAIIEDDSGFGMPMF
Conjugate
HRP
Applications Tested/Suitable for anti-STAG1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-STAG1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-STAG1 antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-STAG1 Monoclonal Antibody – Cat# MBS6155219

Western Blot (WB) of anti-STAG1 antibody

STAG1 monoclonal antibody Western Blot analysis of STAG1 expression in HeLa
Mouse Anti-STAG1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155219

Immunohistochemistry (IHC) of anti-STAG1 antibody

Immunoperoxidase of monoclonal antibody to STAG1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml]
Mouse Anti-STAG1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6155219

Immunofluorescence (IF) of anti-STAG1 antibody

Immunofluorescence of monoclonal antibody to STAG1 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-STAG1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6155219

Testing Data of anti-STAG1 antibody

Detection limit for recombinant GST tagged STAG1 is ~0.03ng/ml as a capture antibody.
Mouse Anti-STAG1 Monoclonal Antibody, Testing Data – Cat# MBS6155219

Other Notes

Small volumes of anti-STAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STAG1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens stromal antigen 1, mRNA
NCBI Official Synonym Full Names
stromal antigen 1
NCBI Official Symbol
STAG1
NCBI Official Synonym Symbols
SA1; SCC3A
Protein Family
NCBI Protein Information
cohesin subunit SA-1

NCBI Summary for STAG1

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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