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TCP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155366

MBS6155366 is an antibody targeting TCP1. Also known as TCP1 (T-complex Protein 1 Subunit alpha, TCP-1-alpha, CCT-alpha, CCT1, CCTA, D6S230E) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TCP1, Monoclonal Antibody
Full Product Name
TCP1 (T-complex Protein 1 Subunit alpha, TCP-1-alpha, CCT-alpha, CCT1, CCTA, D6S230E) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2B2-D6
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TCP1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
60,344 Da
Immunogen
Full length recombinant corresponding to aa1-557 from human TCP1 (AAH00665) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSFGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVIT
Conjugate
HRP
Applications Tested/Suitable for anti-TCP1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-TCP1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TCP1 antibody

TCP1 monoclonal antibody, Western Blot analysis of TCP1 expression in Hela.
Mouse Anti-TCP1 Monoclonal Antibody – Cat# MBS6155366

Immunohistochemistry (IHC) of anti-TCP1 antibody

Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].
Mouse Anti-TCP1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6155366

Immunofluorescence (IF) of anti-TCP1 antibody

Immunofluorescence of monoclonal antibody to TCP1 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-TCP1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6155366

Testing Data of anti-TCP1 antibody

Detection limit for recombinant GST tagged TCP1 is ~0.1ng/ml as a capture antibody.
Mouse Anti-TCP1 Monoclonal Antibody, Testing Data – Cat# MBS6155366

Other Notes

Small volumes of anti-TCP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TCP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens t-complex 1, mRNA
NCBI Official Synonym Full Names
t-complex 1
NCBI Official Symbol
TCP1
NCBI Official Synonym Symbols
CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
Protein Family
NCBI Protein Information
T-complex protein 1 subunit alpha

NCBI Summary for TCP1

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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