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TOM1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155478

MBS6155478 is an antibody targeting TOM1. Also known as TOM1 (Target of Myb Protein 1) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TOM1, Monoclonal Antibody
Full Product Name
TOM1 (Target of Myb Protein 1) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5A3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TOM1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
604700
3D Structure
ModBase 3D Structure for O60784
Immunogen
Partial recombinant corresponding to aa394-491 from TOM1 (NP_005479) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
GLAGALDARQQSTGAIPVTQACLMEDIEQWLSTDVGNDAEEPKGVTSEEFDKFLEERAKAADRLPNLSSPSAEGPPGPPSGPAPRKKTQEKDDDMLFA
Conjugate
HRP

Product Categories/Family for anti-TOM1 antibody

Applications Tested/Suitable for anti-TOM1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-TOM1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TOM1 antibody

Western Blot detection against Immunogen (36.52kD).
Mouse Anti-TOM1 Monoclonal Antibody – Cat# MBS6155478

Western Blot (WB) of anti-TOM1 antibody

TOM1 monoclonal antibody Western Blot analysis of TOM1 expression in K-562
Mouse Anti-TOM1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155478

Western Blot (WB) of anti-TOM1 antibody

Western Blot analysis of TOM1 expression in transfected 293T cell line by TOM1 monoclonal antibody Lane 1: TOM1 transfected lysate (53.8kD). Lane 2: Non-transfected lysate.
Mouse Anti-TOM1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155478

Immunohistochemistry (IHC) of anti-TOM1 antibody

Immunoperoxidase of monoclonal antibody to TOM1 on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3ug/ml]
Mouse Anti-TOM1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6155478

Testing Data of anti-TOM1 antibody

Detection limit for recombinant GST tagged TOM1 is ~0.3ng/ml as a capture antibody.
Mouse Anti-TOM1 Monoclonal Antibody, Testing Data – Cat# MBS6155478

Other Notes

Small volumes of anti-TOM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TOM1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens target of myb1 membrane trafficking protein (TOM1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
target of myb1 membrane trafficking protein
NCBI Official Symbol
TOM1
UniProt Protein Name
Target of Myb protein 1
UniProt Gene Name
TOM1
UniProt Entry Name
TOM1_HUMAN
NCBI Protein Information
target of Myb protein 1

NCBI Summary for TOM1

This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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