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TPM4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155492

MBS6155492 is an antibody targeting TPM4. Also known as TPM4 (Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TPM4, Monoclonal Antibody
Full Product Name
TPM4 (Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4E4-1D2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TPM4.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
32,723 Da
Immunogen
Full length recombinant corresponding to aa1-248 from human TPM4 (AAH37576) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAGLNSLEAVKRKIQALQQQADEAEDRAQGLQRELDGERERREKAEGDVAALNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMEIQEMQLKEAKHIAEEADRKYEEVARKLVILEGELERAEERAEVSELKCGDLEEELKNVTNNLKSLEAASEKYSEKEDKYEEEIKLLSDKLKEAETRAEFAERTVAKLEKTIDDLEEKLAQAKEENVGLHQTLDQTLNELNCI
Conjugate
HRP
Applications Tested/Suitable for anti-TPM4 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-TPM4 antibody
IHC-P: 2ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TPM4 antibody

Western Blot detection against Immunogen (53.02kD).
Mouse Anti-TPM4 Monoclonal Antibody – Cat# MBS6155492

Western Blot (WB) of anti-TPM4 antibody

TPM4 monoclonal antibody. Western Blot analysis of TPM4 expression in Hela.
Mouse Anti-TPM4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155492

Immunohistochemistry (IHC) of anti-TPM4 antibody

Immunoperoxidase of monoclonal antibody to TPM4 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 2ug/ml]
Mouse Anti-TPM4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6155492

Immunofluorescence (IF) of anti-TPM4 antibody

Immunofluorescence of monoclonal antibody to TPM4 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-TPM4 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6155492

Testing Data of anti-TPM4 antibody

Detection limit for recombinant GST tagged TPM4 is ~1ng/ml as a capture antibody.
Mouse Anti-TPM4 Monoclonal Antibody, Testing Data – Cat# MBS6155492

Other Notes

Small volumes of anti-TPM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPM4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens tropomyosin 4, mRNA
NCBI Official Synonym Full Names
tropomyosin 4
NCBI Official Symbol
TPM4
NCBI Official Synonym Symbols
HEL-S-108
Protein Family
NCBI Protein Information
tropomyosin alpha-4 chain

NCBI Summary for TPM4

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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