• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ALPPL2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6156490

MBS6156490 is an antibody targeting ALPPL2. Also known as ALPPL2 (Alkaline Phosphatase, Placental-like, ALP-1, Alkaline Phosphatase Nagao Isozyme, Germ Cell Alkaline Phosphatase, GCAP, Placental Alkaline Phosphatase-like, PLAP-like, ALPPL) (PE). Reactive in… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALPPL2, Monoclonal Antibody
Full Product Name
ALPPL2 (Alkaline Phosphatase, Placental-like, ALP-1, Alkaline Phosphatase Nagao Isozyme, Germ Cell Alkaline Phosphatase, GCAP, Placental Alkaline Phosphatase-like, PLAP-like, ALPPL) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2B3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ALPPL2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
59kDa
OMIM
171810
3D Structure
ModBase 3D Structure for P10696
Immunogen
Partial recombinant corresponding to aa365-454 from human ALPPL2 (NP_112603) with GST tag. MW of the GST tag alone is 26kD.
Conjugate
PE

Product Categories/Family for anti-ALPPL2 antibody

Applications Tested/Suitable for anti-ALPPL2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-ALPPL2 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ALPPL2 antibody

Western Blot detection against Immunogen (35.64kD).
Mouse Anti-ALPPL2 Monoclonal Antibody – Cat# MBS6156490

Western Blot (WB) of anti-ALPPL2 antibody

ALPPL2 monoclonal antibody Western Blot analysis of ALPPL2 expression in A-431.
Mouse Anti-ALPPL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6156490

Western Blot (WB) of anti-ALPPL2 antibody

Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody Lane 1: ALPPL2 transfected lysate (57.5kD). Lane 2: Non-transfected lysate.
Mouse Anti-ALPPL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6156490

Immunohistochemistry (IHC) of anti-ALPPL2 antibody

Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].
Mouse Anti-ALPPL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6156490

Immunoprecipitation (IP) of anti-ALPPL2 antibody

Immunoprecipitation of ALPPL2 transfected lysate using ALPPL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALPPL2 rabbit polyclonal antibody.
Mouse Anti-ALPPL2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6156490

Western Blot (WB) of anti-ALPPL2 antibody

Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-ALPPL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6156490

Other Notes

Small volumes of anti-ALPPL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALPPL2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alkaline phosphatase, germ cell type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, germ cell
NCBI Official Symbol
ALPG
NCBI Official Synonym Symbols
GCAP; ALPPL; ALPPL2
UniProt Protein Name
Alkaline phosphatase, placental-like
UniProt Synonym Protein Names
ALP-1; Alkaline phosphatase Nagao isozyme; Germ cell alkaline phosphatase; GCAP; Placental alkaline phosphatase-like
Protein Family
UniProt Gene Name
ALPPL2
UniProt Synonym Gene Names
ALPPL; GCAP; PLAP-like
UniProt Entry Name
PPBN_HUMAN
NCBI Protein Information
alkaline phosphatase, germ cell type

NCBI Summary for ALPPL2

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for ALPPL2

ALPPL2: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, GPI anchor; EC 3.1.3.1; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - folate biosynthesis

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: plasma membrane

Molecular Function: alkaline phosphatase activity; metal ion binding

Biological Process: dephosphorylation
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000