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EIF2C2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157595

MBS6157595 is an antibody targeting EIF2C2. Also known as EIF2C2 (Protein Argonaute-2, Argonaute2, hAgo2, Eukaryotic Translation Initiation Factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi Domain Protein, PPD, Protein Slicer, AGO2) (PE). Reactive in Human. Host:… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EIF2C2, Monoclonal Antibody
Full Product Name
EIF2C2 (Protein Argonaute-2, Argonaute2, hAgo2, Eukaryotic Translation Initiation Factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi Domain Protein, PPD, Protein Slicer, AGO2) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2E12-1C9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human EIF2C2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
93,620 Da
Immunogen
Full length recombinant corresponding to aa1-377 from human EIF2C2 (AAH07633) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVD
Conjugate
PE
Applications Tested/Suitable for anti-EIF2C2 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-EIF2C2 antibody
IHC-P: 3ug/ml
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EIF2C2 antibody

Western Blot detection against Immunogen (68.4kD).
Mouse Anti-EIF2C2 Monoclonal Antibody – Cat# MBS6157595

Immunohistochemistry (IHC) of anti-EIF2C2 antibody

Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml]
Mouse Anti-EIF2C2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6157595

Immunofluorescence (IF) of anti-EIF2C2 antibody

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-EIF2C2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6157595

Western Blot (WB) of anti-EIF2C2 antibody

Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-EIF2C2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157595

Other Notes

Small volumes of anti-EIF2C2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2C2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 2C, 2, mRNA
NCBI Official Synonym Full Names
argonaute 2, RISC catalytic component
NCBI Official Symbol
AGO2
NCBI Official Synonym Symbols
Q10; EIF2C2
NCBI Protein Information
protein argonaute-2

NCBI Summary for EIF2C2

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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