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EIF2S1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157597

MBS6157597 is an antibody targeting EIF2S1. Also known as EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, eIF2 alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A, Eukaryotic Translation Initiation Factor 2 Subunit 1) (PE). Reactive in Human.… More details

$1140 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EIF2S1, Monoclonal Antibody
Full Product Name
EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, eIF2 alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A, Eukaryotic Translation Initiation Factor 2 Subunit 1) (PE)
Product Gene Name
anti-EIF2S1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3H12-C11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human EIF2S1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
36,112 Da
Immunogen
Full length recombinant corresponding to aa1-315 from human EIF2S1 (AAH02513.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED
Conjugate
PE

Product Categories/Family for anti-EIF2S1 antibody

Antibodies; Translation Initiation Factors
Applications Tested/Suitable for anti-EIF2S1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-EIF2S1 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EIF2S1 antibody

Western Blot detection against Immunogen (60.39kD).
Mouse Anti-EIF2S1 Monoclonal Antibody – Cat# MBS6157597

Western Blot (WB) of anti-EIF2S1 antibody

Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody. Lane 1: EIF2S1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.
Mouse Anti-EIF2S1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157597

Immunofluorescence (IF) of anti-EIF2S1 antibody

Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10ug/ml].
Mouse Anti-EIF2S1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6157597

Testing Data of anti-EIF2S1 antibody

Detection limit for recombinant GST tagged EIF2S1 is ~0.3ng/ml as a capture antibody.
Mouse Anti-EIF2S1 Monoclonal Antibody, Testing Data – Cat# MBS6157597

Western Blot (WB) of anti-EIF2S1 antibody

Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-EIF2S1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157597

Western Blot (WB) of anti-EIF2S1 antibody

EIF2S1 monoclonal antibody. Western Blot analysis of EIF2S1 expression in Hela NE.
Mouse Anti-EIF2S1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157597

Western Blot (WB) of anti-EIF2S1 antibody

EIF2S1 monoclonal antibody, Western Blot analysis of EIF2S1 expression in HeLa.
Mouse Anti-EIF2S1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157597

Product References and Citations for anti-EIF2S1 antibody

1. IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR. Weinlich S, Huttelmaier S, Schierhorn A, Behrens SE, Ostareck-Lederer A, Ostareck DH.RNA. 2009 Aug;15(8):1528-42. Epub 2009 Jun 18.

Other Notes

Small volumes of anti-EIF2S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2S1. It may not necessarily be applicable to this product.

NCBI GI #
33876800
NCBI GeneID
1965
NCBI Accession #
BC002513
NCBI Related Accession #
AAH02513.1
UniProt Related Accession #
P05198
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, mRNA
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol
EIF2S1
NCBI Official Synonym Symbols
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Protein Family
Eukaryotic translation initiation factor
NCBI Protein Information
eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha

NCBI Summary for EIF2S1

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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Research Articles on EIF2S1

1. eIF1, eIF1A, eIF3j, and the eIF2-GTP-Met-tRNAi ternary complex stably bind to the 43 S preinitiation complex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EIF2S1 antibody

 Reference Product  PubMed Publications
 ATF4 antibody  >4 publications with EIF2S1 and ATF4
 EIF2AK4 antibody  >2 publications with EIF2S1 and EIF2AK4
 EIF2AK2 antibody  >2 publications with EIF2S1 and EIF2AK2
 EIF2AK3 antibody  >2 publications with EIF2S1 and EIF2AK3
 EIF5 antibody  >1 publications with EIF2S1 and EIF5
 EIF2S2 antibody  >1 publications with EIF2S1 and EIF2S2

Pathways associated with anti-EIF2S1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram

Diseases associated with anti-EIF2S1 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with EIF2S1 and Cell Transformation, Neoplastic
 Hyperglycemia Antibodies  >1 publications with EIF2S1 and Hyperglycemia
 Glucose Intolerance Antibodies  >1 publications with EIF2S1 and Glucose Intolerance
 Disease Models, Animal Antibodies  >1 publications with EIF2S1 and Disease Models, Animal
 Inflammation Antibodies  >1 publications with EIF2S1 and Inflammation

Organs/Tissues associated with anti-EIF2S1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with EIF2S1 and Brain
 Heart Antibodies  >1 publications with EIF2S1 and Heart
 Muscle Antibodies  >1 publications with EIF2S1 and Muscle
 Pancreas Antibodies  >1 publications with EIF2S1 and Pancreas
 Blood Antibodies  >1 publications with EIF2S1 and Blood
 Placenta Antibodies  >1 publications with EIF2S1 and Placenta
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