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EIF4G3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157609

MBS6157609 is an antibody targeting EIF4G3. Also known as EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3, eIF-4-gamma 3, eIF-4G 3, eIF4G 3, eIF-4-gamma II, eIF4GII) (PE). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EIF4G3, Monoclonal Antibody
Full Product Name
EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3, eIF-4-gamma 3, eIF-4G 3, eIF4G 3, eIF-4-gamma II, eIF4GII) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1H5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human EIF4G3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
146,871 Da
Immunogen
Full length recombinant corresponding to aa1-516 from EIF4G3 (AAH30578) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKK
Conjugate
PE
Applications Tested/Suitable for anti-EIF4G3 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-EIF4G3 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EIF4G3 antibody

Western blot analysis of EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF4G3 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-EIF4G3 Monoclonal Antibody – Cat# MBS6157609

Testing Data of anti-EIF4G3 antibody

Detection limit for recombinant GST tagged EIF4G3 is ~1ng/ml as a capture antibody.
Mouse Anti-EIF4G3 Monoclonal Antibody, Testing Data – Cat# MBS6157609

Immunohistochemistry (IHC) of anti-EIF4G3 antibody

Immunoperoxidase of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 3ug/ml].
Mouse Anti-EIF4G3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6157609

Western Blot (WB) of anti-EIF4G3 antibody

Western Blot analysis of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody Lane 1: EIF4G3 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-EIF4G3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157609

Western Blot (WB) of anti-EIF4G3 antibody

EIF4G3 monoclonal antibody Western Blot analysis of EIF4G3 expression in MCF-7.
Mouse Anti-EIF4G3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157609

Other Notes

Small volumes of anti-EIF4G3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EIF4G3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens eukaryotic translation initiation factor 4 gamma, 3, mRNA
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4 gamma 3
NCBI Official Symbol
EIF4G3
NCBI Official Synonym Symbols
eIF4G 3; eIF4GII; eIF-4G 3
NCBI Protein Information
eukaryotic translation initiation factor 4 gamma 3

NCBI Summary for EIF4G3

The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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