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ELA1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157615

MBS6157615 is an antibody targeting ELA1. Also known as ELA1 (Chymotrypsin-like Elastase Family Member 1, Elastase-1, Pancreatic Elastase 1, CELA1) (PE). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ELA1, Monoclonal Antibody
Full Product Name
ELA1 (Chymotrypsin-like Elastase Family Member 1, Elastase-1, Pancreatic Elastase 1, CELA1) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4H5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ELA1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
27,798 Da
OMIM
130120
3D Structure
ModBase 3D Structure for Q9UNI1
Immunogen
Partial recombinant corresponding to aa159-257 from human ELA1 (NP_001962) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
LAQTLQQAYLPSVDYAICSSSSYWGSTVKNTMVCAGGDGVRSGCQGDSGGPLHCLVNGKYSVHGVTSFVSSRGCNVSRKPTVFTQVSAYISWINNVIAS
Conjugate
PE

Product Categories/Family for anti-ELA1 antibody

Applications Tested/Suitable for anti-ELA1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-ELA1 antibody
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ELA1 antibody

Western Blot detection against Immunogen (36.63kD).
Mouse Anti-ELA1 Monoclonal Antibody – Cat# MBS6157615

Immunohistochemistry (IHC) of anti-ELA1 antibody

Immunoperoxidase of monoclonal antibody to ELA1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].
Mouse Anti-ELA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6157615

Testing Data of anti-ELA1 antibody

Detection limit for recombinant GST tagged ELA1 is 3ng/ml as a capture antibody.
Mouse Anti-ELA1 Monoclonal Antibody, Testing Data – Cat# MBS6157615

Other Notes

Small volumes of anti-ELA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ELA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chymotrypsin-like elastase family member 1
NCBI Official Synonym Full Names
chymotrypsin-like elastase family, member 1
NCBI Official Symbol
CELA1
NCBI Official Synonym Symbols
ELA1
UniProt Protein Name
Chymotrypsin-like elastase family member 1
UniProt Synonym Protein Names
Elastase-1; Pancreatic elastase 1
Protein Family
UniProt Gene Name
CELA1
UniProt Synonym Gene Names
ELA1
UniProt Entry Name
CELA1_HUMAN
NCBI Protein Information
chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic

NCBI Summary for ELA1

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
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UniProt Comments for ELA1

CELA1: Acts upon elastin. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.36

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; proteolysis; inflammatory response; regulation of cell proliferation; post-embryonic development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ELA1 antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >1 publications with ELA1 and Fatty Liver
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