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Endoglin (END) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157639

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95kD subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-B)… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endoglin, Monoclonal Antibody
Full Product Name
Endoglin (CD105, ENG, END) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4C11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human ENG.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
67,542 Da
Immunogen
Full length recombinant corresponding to aa27-658 from human ENG (AAH14271) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TVHCDLQPVGPERDEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGSQDQEVHRTVFMRLNIISPDLSGCTSKGLVLPAVLGITFGAFLIGALLTAALWYIYSHTRSPSKREPVVAVAAPASSESSSTNHSIGSTQSTPCSTSSMA
Conjugate
PE

Product Categories/Family for anti-END antibody

Applications Tested/Suitable for anti-END antibody
FLISA, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-END antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-END antibody

Western Blot detection against Immunogen (95.26kD).
Mouse Anti-END Monoclonal Antibody – Cat# MBS6157639

Western Blot (WB) of anti-END antibody

Western Blot analysis of ENG expression in transfected 293T cell line by 126323. Lane 1: ENG transfected lysate (70.6kD). Lane 2: Non-transfected lysate.
Mouse Anti-END Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157639

Immunoprecipitation (IP) of anti-END antibody

Immunoprecipitation of ENG transfected lysate using 126323 and Protein A Magnetic Bead and immunoblotted with ENG rabbit polyclonal antibody.
Mouse Anti-END Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6157639

Testing Data of anti-END antibody

Detection limit for recombinant GST tagged ENG is ~0.3ng/ml as a capture antibody.
Mouse Anti-END Monoclonal Antibody, Testing Data – Cat# MBS6157639

Other Notes

Small volumes of anti-END antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for END purchase

MBS6157639 is an antibody targeting END. Also known as Endoglin (CD105, ENG, END) (PE). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for END. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens endoglin, mRNA
NCBI Official Synonym Full Names
endoglin
NCBI Official Symbol
ENG
NCBI Official Synonym Symbols
END; HHT1; ORW1
Protein Family
NCBI Protein Information
endoglin

NCBI Summary for END

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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