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FGL2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6157811

MBS6157811 is an antibody targeting FGL2. Also known as FGL2 (Fibroleukin, Fibrinogen-like Protein 2, pT49) (PE). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FGL2, Monoclonal Antibody
Full Product Name
FGL2 (Fibroleukin, Fibrinogen-like Protein 2, pT49) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6D9
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human FGL2. Species Crossreactivity: mouse and rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
48
OMIM
605351
3D Structure
ModBase 3D Structure for Q14314
Immunogen
Partial recombinant corresponding to aa24-123 from human FGL2 (NP_006673) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NNETEEIKDERAKDVCPVRLESRGKCEEAGECPYQVSLPPLTIQLPKQFSRIEEVFKEVQNLKEIVNSLKKSCQDCKLQADDNGDPGRNGLLLPSTGAPG
Conjugate
PE

Product Categories/Family for anti-FGL2 antibody

Applications Tested/Suitable for anti-FGL2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-FGL2 antibody
IHC-P: 0.5ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-FGL2 antibody

FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in Raw 264.7.
Mouse Anti-FGL2 Monoclonal Antibody – Cat# MBS6157811

Western Blot (WB) of anti-FGL2 antibody

Western Blot analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody Lane 1: FGL2 transfected lysate (50kD). Lane 2: Non-transfected lysate.
Mouse Anti-FGL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157811

Immunohistochemistry (IHC) of anti-FGL2 antibody

Immunoperoxidase of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5ug/ml].
Mouse Anti-FGL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6157811

Immunoprecipitation (IP) of anti-FGL2 antibody

Immunoprecipitation of FGL2 transfected lysate using FGL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FGL2 rabbit polyclonal antibody.
Mouse Anti-FGL2 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6157811

Testing Data of anti-FGL2 antibody

Detection limit for recombinant GST tagged FGL2 is ~0.1ng/ml as a capture antibody.
Mouse Anti-FGL2 Monoclonal Antibody, Testing Data – Cat# MBS6157811

Western Blot (WB) of anti-FGL2 antibody

FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in PC-12.
Mouse Anti-FGL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157811

Western Blot (WB) of anti-FGL2 antibody

Western Blot detection against Immunogen (37.11kD).
Mouse Anti-FGL2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6157811

Product References and Citations for anti-FGL2 antibody

1. FGL2/Fibroleukin Mediates Hepatic Reperfusion Injury by Induction of Sinusoidal Endothelial Cell and Hepatocyte Apoptosis in Mice. Selzner N, Liu H, Boehnert MU, Adeyi OA, Shalev I, Bartczak AM, Xue-Zhong M, Manuel J, Rotstein OD, McGilvray ID, Grant DR, Phillips MJ, Levy GA, Selzner M.J Hepatol. 2011 Jul 11. 2. The FGL2/fibroleukin prothrombinase is involved in alveolar macrophage activation in COPD through the MAPK pathway. Liu Y, Xu S, Xiao F, Xiong Y, Wang X, Gao S, Yan W, Ning Q.Biochem Biophys Res Commun. 2010 May 7. 3. Targeted Deletion of fgl2 Leads to Impaired Regulatory T Cell Activity and Development of Autoimmune Glomerulonephritis1. Shalev I, Liu H, Koscik C, Bartczak A, Javadi M, Wong KM, Maknojia A, He W, Liu MF, Diao J, Winter E, Manuel J, McCarthy D, Cattral M, Gommerman J, Clark DA, Phillips MJ, Gorczynski RR, Zhang L, Downey G, Grant D, Cybulsky MI, Levy G.J Immunol. 2008 Jan 1;180(1):249-60. 4. Expression of Prothrombin and Protease Activated Receptors in Human Myometrium During Pregnancy and Labor. O'brien M, Morrison JJ, Smith TJ.Biol Reprod. 2008 Jan;78(1):20-6. Epub 2007 Sep 26.

Other Notes

Small volumes of anti-FGL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FGL2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
fibroleukin
NCBI Official Synonym Full Names
fibrinogen like 2
NCBI Official Symbol
FGL2
NCBI Official Synonym Symbols
T49; pT49
UniProt Protein Name
Fibroleukin
UniProt Synonym Protein Names
Fibrinogen-like protein 2; pT49
Protein Family
UniProt Gene Name
FGL2
UniProt Entry Name
FGL2_HUMAN
NCBI Protein Information
fibroleukin

NCBI Summary for FGL2

The protein encoded by this gene is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites. [provided by RefSeq, Jul 2008]
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UniProt Comments for FGL2

FGL2: May play a role in physiologic lymphocyte functions at mucosal sites.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: fibrinogen complex

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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