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IgG, H&L, X-Adsorbed Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS615803

MBS615803 is an antibody targeting IgG, H&L, X-Adsorbed. Also known as IgG, H&L, X-Adsorbed (HRP). Reactive in Rat. Host: Rabbit. More details

$775 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IgG, H&L, X-Adsorbed, Polyclonal Antibody
Full Product Name
IgG, H&L, X-Adsorbed (HRP)
Product Synonym Names
Anti -IgG, H&L, X-Adsorbed (HRP)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography followed by solid phase adsorption to remove any unwanted reactivities.
Specificity
Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, rat IgG and rat Serum. No reaction was observed against human serum proteins.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Rat IgG whole molecule.
Applications Tested/Suitable for anti-IgG, H&L, X-Adsorbed antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
Suitable forfor use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and immunohistochemistry.
Dilution: ELISA: 1:2000-1:10,000. Use 1ug of Rat IgG in a standard capture ELISA.
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to bedetermined by researcher.

Western Blot (WB) of anti-IgG, H&L, X-Adsorbed antibody

Western Blot of MBS615803 Lane M: 3ul Molecular Ladder. Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) Fragment Lane 3: Rat IgG Fab Fragment Lane 4: Rat IgM Whole Molecule Lane 5: Rat Serum All samples were reduced. Load: 50ng per lane.
Rabbit Anti-IgG, H&L, X-Adsorbed Polyclonal Antibody – Cat# MBS615803

Other Notes

Small volumes of anti-IgG, H&L, X-Adsorbed antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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