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MRC1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6158886

MBS6158886 is an antibody targeting MRC1. Also known as MRC1 (Macrophage Mannose Receptor 1, MMR, C-type Lectin Domain Family 13 Member D, C-type Lectin Domain Family 13 Member D-like, Macrophage Mannose Receptor 1-like Protein 1, CD206, CLEC13D, CLEC13DL,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MRC1, Monoclonal Antibody
Full Product Name
MRC1 (Macrophage Mannose Receptor 1, MMR, C-type Lectin Domain Family 13 Member D, C-type Lectin Domain Family 13 Member D-like, Macrophage Mannose Receptor 1-like Protein 1, CD206, CLEC13D, CLEC13DL, MRC1L1) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5C11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MRC1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
153618
3D Structure
ModBase 3D Structure for P22897
Immunogen
Partial recombinant corresponding to aa22-130 from human MRC1 (NP_002429) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TRQFLIYNEDHKRCVDAVSPSAVQTAACNQDAESQKFRWVSESQIMSVAFKLCLGVPSKTDWVAITLYACDSKSEFQKWECKNDTLLGIKGEDLFFNYGNRQEKNIMLY
Conjugate
PE

Product Categories/Family for anti-MRC1 antibody

Applications Tested/Suitable for anti-MRC1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-MRC1 antibody
IHC-P: 1.5ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MRC1 antibody

Western Blot detection against Immunogen (37.73kD).
Mouse Anti-MRC1 Monoclonal Antibody – Cat# MBS6158886

Western Blot (WB) of anti-MRC1 antibody

MRC1 monoclonal antibody. Western Blot analysis of MRC1 expression in human pancreas.
Mouse Anti-MRC1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6158886

Immunohistochemistry (IHC) of anti-MRC1 antibody

Immunoperoxidase of monoclonal antibody to MRC1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5ug/ml]
Mouse Anti-MRC1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6158886

Testing Data of anti-MRC1 antibody

Detection limit for recombinant GST tagged MRC1 is ~0.03ng/ml as a capture antibody.
Mouse Anti-MRC1 Monoclonal Antibody, Testing Data – Cat# MBS6158886

Product References and Citations for anti-MRC1 antibody

1. Xanthogranulomatous cholecystitis: A clinicopathological study of its association with gallbladder carcinoma. Zhuang PY, Zhu MJ, Wang JD, Zhou XP, Quan ZW, Shen J.J Dig Dis. 2012 Sep 21. doi: 10.1111/j.1751-2980.2012.00645.x. 2. Coronary Atherosclerosis Is Associated With Macrophage Polarization in Epicardial Adipose Tissue. Hirata Y, Tabata M, Kurobe H, Motoki T, Akaike M, Nishio C, Higashida M, Mikasa H, Nakaya Y, Takanashi S, Igarashi T, Kitagawa T, Sata M.J Am Coll Cardiol. 2011 Jul 12;58(3):248-55.

Other Notes

Small volumes of anti-MRC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MRC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
macrophage mannose receptor 1
NCBI Official Synonym Full Names
mannose receptor C-type 1
NCBI Official Symbol
MRC1
NCBI Official Synonym Symbols
MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
UniProt Protein Name
Macrophage mannose receptor 1
UniProt Synonym Protein Names
C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1
UniProt Gene Name
MRC1
UniProt Synonym Gene Names
CLEC13D; CLEC13DL; MRC1L1; MMR
UniProt Entry Name
MRC1_HUMAN
NCBI Protein Information
macrophage mannose receptor 1

NCBI Summary for MRC1

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]
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UniProt Comments for MRC1

MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; endosome membrane

Molecular Function: mannose binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: receptor-mediated endocytosis; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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