HSPB1 Antibody – Rabbit Polyclonal

Human Hsp27 belongs to the phylogenically conserved small heat shock protein (smHsp) family, which includes mouse Hsp25, alphaA-crystallins and alphaB-crystallins. Members of this protein superfamily from the animal, plant and microbiotic kingdoms share the so-called alpha-crystallin domain of approximately 90 amino acid residues which is bounded by variable N-and C-terminal extensions (1). Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 of Hsp27 protein monomers in cells and data suggest that these large oligomers have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur (2). Hsp27 is phosphorylated on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2/3 and the stress-activated protein kinase SAPK2 (p38) (3,4). Studies on cells stimulated by a variety of mitogenic and stress factors suggest that phosphorylation of Hsp27 occurs as an early prominent event and that phosphorylation induced changes in the ultrastructure of Hsp27 may regulate its biochemical activities. The state of phosphorylation and oligomerization of Hsp27 may regulate microfilament organization as shown by studies demonstrating that only the nonphosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization (5). Hsp27 is believed to protect cells by enhancing cellular glutathione levels as indicated by elevated glutathione levels in cells overexpressing Hsp27. Studies using wild-type Hsp27 and mutants in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, demonstrate that cellular glutathione levels depend on the oligomerization of Hsp27 with only the large oligomeric forms of Hsp27 capable of protecting cells by enhancing glutathione levels (6).

Small volumes of anti-HSPB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS615913 is an antibody targeting HSPB1. Also known as Hsp27, phosphorylated (Ser78). Reactive in Bovine, Guinea Pig, Human, Monkey. Host: Rabbit.