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PEPD Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6159392

MBS6159392 is an antibody targeting PEPD. Also known as PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) (PE). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PEPD, Monoclonal Antibody
Full Product Name
PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D5-H3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human PEPD.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-494 from human PEPD (AAH15027) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVAEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQEPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFLNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK
Conjugate
PE

Product Categories/Family for anti-PEPD antibody

Applications Tested/Suitable for anti-PEPD antibody
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PEPD antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PEPD antibody

Western Blot detection against Immunogen (79.97kD).
Mouse Anti-PEPD Monoclonal Antibody – Cat# MBS6159392

Western Blot (WB) of anti-PEPD antibody

PEPD monoclonal antibody Western Blot analysis of PEPD expression in HepG2.
Mouse Anti-PEPD Monoclonal Antibody, Western Blot (WB) – Cat# MBS6159392

Western Blot (WB) of anti-PEPD antibody

Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody. Lane 1: PEPD transfected lysate (Predicted MW: 54.6kD). Lane 2: Non-transfected lysate
Mouse Anti-PEPD Monoclonal Antibody, Western Blot (WB) – Cat# MBS6159392

Immunofluorescence (IF) of anti-PEPD antibody

Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10ug/ml]
Mouse Anti-PEPD Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6159392

Immunoprecipitation (IP) of anti-PEPD antibody

Immunoprecipitation of PEPD transfected lysate using PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.
Mouse Anti-PEPD Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6159392

Testing Data of anti-PEPD antibody

Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.
Mouse Anti-PEPD Monoclonal Antibody, Testing Data – Cat# MBS6159392

Other Notes

Small volumes of anti-PEPD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PEPD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens peptidase D, mRNA
NCBI Official Synonym Full Names
peptidase D
NCBI Official Symbol
PEPD
NCBI Official Synonym Symbols
PROLIDASE
NCBI Protein Information
xaa-Pro dipeptidase

NCBI Summary for PEPD

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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