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TFAP4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6160688

MBS6160688 is an antibody targeting TFAP4. Also known as TFAP4 (BHLHC41, Transcription Factor AP-4, Activating Enhancer-binding Protein 4, Class C Basic Helix-loop-helix Protein 41) (PE). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TFAP4, Monoclonal Antibody
Full Product Name
TFAP4 (BHLHC41, Transcription Factor AP-4, Activating Enhancer-binding Protein 4, Class C Basic Helix-loop-helix Protein 41) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
7A10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TFAP4.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
39kDa
OMIM
600743
3D Structure
ModBase 3D Structure for Q01664
Immunogen
Partial recombinant corresponding to aa93-193 from human TFAP4 (NP_003214) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
AEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIA
Conjugate
PE

Product Categories/Family for anti-TFAP4 antibody

Applications Tested/Suitable for anti-TFAP4 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-TFAP4 antibody
IHC-P: 1ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TFAP4 antibody

TFAP4 monoclonal antibody Western Blot analysis of TFAP4 expression in Hela NE.
Mouse Anti-TFAP4 Monoclonal Antibody – Cat# MBS6160688

Western Blot (WB) of anti-TFAP4 antibody

Western Blot analysis of TFAP4 expression in transfected 293T cell line by TFAP4 monoclonal antibody. Lane 1: TFAP4 transfected lysate (38.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-TFAP4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6160688

Immunohistochemistry (IHC) of anti-TFAP4 antibody

Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml]
Mouse Anti-TFAP4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6160688

Testing Data of anti-TFAP4 antibody

Detection limit for recombinant GST tagged TFAP4 is ~0.1ng/ml as a capture antibody.
Mouse Anti-TFAP4 Monoclonal Antibody, Testing Data – Cat# MBS6160688

Western Blot (WB) of anti-TFAP4 antibody

Western blot analysis of TFAP4 over-expressed 293 cell line, cotransfected with TFAP4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TFAP4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.
Mouse Anti-TFAP4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6160688

Other Notes

Small volumes of anti-TFAP4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TFAP4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor AP-4
NCBI Official Synonym Full Names
transcription factor AP-4
NCBI Official Symbol
TFAP4
NCBI Official Synonym Symbols
AP-4; bHLHc41
UniProt Protein Name
Transcription factor AP-4
UniProt Synonym Protein Names
Activating enhancer-binding protein 4; Class C basic helix-loop-helix protein 41; bHLHc41
Protein Family
UniProt Gene Name
TFAP4
UniProt Synonym Gene Names
BHLHC41; bHLHc41
UniProt Entry Name
TFAP4_HUMAN
NCBI Protein Information
transcription factor AP-4

NCBI Summary for TFAP4

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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