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Transcription Factor Ec (TFEC) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6160696

MBS6160696 is an antibody targeting TFEC. Also known as Transcription Factor EC (TFEC, TFE-C, TCFEC, Class E Basic Helix-loop-helix Protein 34, bHLHe34, Transcription Factor EC-like, TFECL, TFEC-L, hTFEC-L) (PE). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Transcription Factor EC (TFEC), Monoclonal Antibody
Full Product Name
Transcription Factor EC (TFEC, TFE-C, TCFEC, Class E Basic Helix-loop-helix Protein 34, bHLHe34, Transcription Factor EC-like, TFECL, TFEC-L, hTFEC-L) (PE)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4F11
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TFEC.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa1-89 from TFEC (NP_001018068) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTLDHQIINPTLKWSQPAVPSGGPLVQHAHTTLDSDAGLTENPLTKLLAIGKEDDNAQWHLSGSILDVYSGEQGISPINMGLTSASCPS*
Conjugate
PE
Applications Tested/Suitable for anti-TFEC antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-TFEC antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TFEC antibody

Western Blot detection against Immunogen (35.9kD).
Mouse Anti-TFEC Monoclonal Antibody – Cat# MBS6160696

Western Blot (WB) of anti-TFEC antibody

Western Blot analysis of TFEC expression in transfected 293T cell line by TFEC monoclonal antibody Lane 1: TFEC transfected lysate (22.7kD). Lane 2: Non-transfected lysate.
Mouse Anti-TFEC Monoclonal Antibody, Western Blot (WB) – Cat# MBS6160696

Testing Data of anti-TFEC antibody

Detection limit for recombinant GST tagged TFEC is 0.1ng/ml as a capture antibody.
Mouse Anti-TFEC Monoclonal Antibody, Testing Data – Cat# MBS6160696

Other Notes

Small volumes of anti-TFEC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TFEC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens transcription factor EC (TFEC), transcript variant 2, mRNA
NCBI Official Synonym Full Names
transcription factor EC
NCBI Official Symbol
TFEC
NCBI Official Synonym Symbols
TCFEC; TFE-C; TFECL; TFEC-L; bHLHe34; hTFEC-L
Protein Family
NCBI Protein Information
transcription factor EC

NCBI Summary for TFEC

This gene encodes a member of the micropthalmia (MiT) family of basic helix-loop-helix leucine zipper transcription factors. MiT transcription factors regulate the expression of target genes by binding to E-box recognition sequences as homo- or heterodimers, and play roles in multiple cellular processes including survival, growth and differentiation. The encoded protein is a transcriptional activator of the nonmuscle myosin II heavy chain-A gene, and may also co-regulate target genes in osteoclasts as a heterodimer with micropthalmia-associated transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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