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ERN1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6162721

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as… More details

$995 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ERN1, Monoclonal Antibody
Full Product Name
ERN1 (Endoplasmic Reticulum to Nucleus Signaling 1, FLJ30999, IRE1, IRE1P, MGC163277, MGC163279) (AP)
Product Gene Name
Product Synonym Names
Endoplasmic Reticulum to Nucleus Signaling 1; FLJ30999; IRE1; IRE1P; MGC163277; MGC163279
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3D1
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes ERN1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
ERN1 (AAC25991, 401aa-500aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHP
Conjugate
AP

Product Categories/Family for anti-ERN1 antibody

Applications Tested/Suitable for anti-ERN1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ERN1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ERN1 antibody

ERN1 monoclonal antibody (M06), clone 3D1 Western Blot analysis of ERN1 expression in HeLa.
Mouse Anti-ERN1 Monoclonal Antibody – Cat# MBS6162721

Testing Data of anti-ERN1 antibody

Detection limit for recombinant GST tagged ERN1 is approximately 0.3ng/ml as a capture antibody.
Mouse Anti-ERN1 Monoclonal Antibody, Testing Data – Cat# MBS6162721

Immunohistochemistry (IHC) of anti-ERN1 antibody

Immunoperoxidase of monoclonal antibody to ERN1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml]
Mouse Anti-ERN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6162721

Immunohistochemistry (IHC) of anti-ERN1 antibody

Immunoperoxidase of monoclonal antibody to ERN1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml]
Mouse Anti-ERN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6162721

Other Notes

Small volumes of anti-ERN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ERN1 purchase

MBS6162721 is an antibody targeting ERN1. Also known as ERN1 (Endoplasmic Reticulum to Nucleus Signaling 1, FLJ30999, IRE1, IRE1P, MGC163277, MGC163279) (AP). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ERN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
protein kinase/endoribonulcease
NCBI Official Synonym Full Names
endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol
ERN1
NCBI Official Synonym Symbols
IRE1; IRE1P; IRE1a; hIRE1p
NCBI Protein Information
serine/threonine-protein kinase/endoribonuclease IRE1

NCBI Summary for ERN1

This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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