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KLF12 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6163819

Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLF12, Monoclonal Antibody
Full Product Name
KLF12 (Kruppel-like Factor 12, AP-2rep, AP2REP, HSPC122) (AP)
Product Gene Name
Product Synonym Names
Kruppel-like Factor 12; AP-2rep; AP2REP; HSPC122
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
30000
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes KLF12.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
46.6 kDa (425aa)
OMIM
607531
3D Structure
ModBase 3D Structure for Q9Y4X4
Immunogen
KLF12 (NP_009180, 1aa-90aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MNIHMKRKTIKNINTFENRMLMLDGMPAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKGEPPEDSLSVDHFQTQTEPVDLSINKAR
Conjugate
AP
Applications Tested/Suitable for anti-KLF12 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-KLF12 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-KLF12 antibody

Western Blot analysis of KLF12 expression in transfected 293T cell line by KLF12 monoclonal antibody (M01), clone 3E4.

Lane 1: KLF12 transfected lysate(44.2 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-KLF12 Monoclonal Antibody – Cat# MBS6163819

Other Notes

Small volumes of anti-KLF12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF12 purchase

MBS6163819 is an antibody targeting KLF12. Also known as KLF12 (Kruppel-like Factor 12, AP-2rep, AP2REP, HSPC122) (AP). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Krueppel-like factor 12
NCBI Official Synonym Full Names
Kruppel like factor 12
NCBI Official Symbol
KLF12
NCBI Official Synonym Symbols
AP2REP; AP-2rep; HSPC122
UniProt Protein Name
Krueppel-like factor 12
UniProt Synonym Protein Names
Transcriptional repressor AP-2rep
Protein Family
UniProt Gene Name
KLF12
UniProt Synonym Gene Names
AP2REP
UniProt Entry Name
KLF12_HUMAN
NCBI Protein Information
Krueppel-like factor 12

UniProt Comments for KLF12

KLF12: Confers strong transcriptional repression to the AP-2- alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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