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C-Fos Oncoprotein Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616430

c-fos is a 55kD nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures. More details

$1010 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
c-fos Oncoprotein, Polyclonal Antibody
Full Product Name
c-fos Oncoprotein (Fos)
Product Synonym Names
Anti -c-fos Oncoprotein (Fos)
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 10mM sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Specificity
Recognizes human c-fos. Species Crossreactivity: Rat
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala- Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.

Product Categories/Family for anti-c-fos Oncoprotein antibody

Antibodies; Abs to Oncoproteins
Applications Tested/Suitable for anti-c-fos Oncoprotein antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-c-fos Oncoprotein antibody
Suiatble for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. Detects a band at 55-60kD on immunoblots of stimulated HeLa and HL-60 lysates.
Immunocytochemistry: Has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.

Other Notes

Small volumes of anti-c-fos Oncoprotein antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-fos Oncoprotein purchase

MBS616430 is an antibody targeting c-fos Oncoprotein. Also known as c-fos Oncoprotein (Fos). Reactive in Human, Rat. Host: Sheep.
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