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NPM2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6165538

MBS6165538 is an antibody targeting NPM2. Also known as NPM2 (Nucleophosmin/Nucleoplasmin, 2, MGC78655) (AP). Host: Mouse. Mouse monoclonal antibody raised against a full-length recombinant NPM2. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NPM2, Monoclonal Antibody
Full Product Name
NPM2 (Nucleophosmin/Nucleoplasmin, 2, MGC78655) (AP)
Product Gene Name
Product Synonym Names
Nucleophosmin/Nucleoplasmin; 2; MGC78655
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
5.0e+9
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes NPM2.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
24,152 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (21882354..21894408). Location: 8p21.3
OMIM
608073
3D Structure
ModBase 3D Structure for Q86SE8
Immunogen
NPM2 (NP_877724.1, 1aa-214aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MNLSSASSTEEKAVTTVLWGCELSQERRTWTFRPQLEGKQSCRLLLHTICLGEKAKEEMHRVEILPPANQEDKKMQPVTIASLQASVLPMVSMVGVQLSPPVTFQLRAGSGPVFLSGQERYEASDLTWEEEEEEEGEEEEEEEEDDEDEDADISLEEQSPVKQVKRLVPQKQASVAKKKKLEKEEEEIRASVRDKSPVKKAKATARAKKPGFKK
Conjugate
AP

Product Categories/Family for anti-NPM2 antibody

Applications Tested/Suitable for anti-NPM2 antibody
Western Blot (WB)
Application Notes for anti-NPM2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NPM2 antibody

Western Blot analysis of NPM2 expression in transfected 293T cell line by NPM2 monoclonal antibody (M03), clone 5E9.

Lane 1: NPM2 transfected lysate (Predicted MW: 24.2 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-NPM2 Monoclonal Antibody – Cat# MBS6165538

Testing Data of anti-NPM2 antibody

Detection limit for recombinant GST tagged NPM2 is 3 ng/ml as a capture antibody.
Mouse Anti-NPM2 Monoclonal Antibody, Testing Data – Cat# MBS6165538

Immunofluorescence (IF) of anti-NPM2 antibody

Immunofluorescence of monoclonal antibody to NPM2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-NPM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6165538

Immunofluorescence (IF) of anti-NPM2 antibody

Immunofluorescence of monoclonal antibody to NPM2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-NPM2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6165538

Other Notes

Small volumes of anti-NPM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NPM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nucleoplasmin-2
NCBI Official Synonym Full Names
nucleophosmin/nucleoplasmin 2
NCBI Official Symbol
NPM2
UniProt Protein Name
Nucleoplasmin-2
Protein Family
UniProt Gene Name
NPM2
UniProt Entry Name
NPM2_HUMAN
NCBI Protein Information
nucleoplasmin-2

UniProt Comments for NPM2

NPM2: Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization. Belongs to the nucleoplasmin family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: nuclear chromatin; nucleus

Molecular Function: enzyme binding; nucleic acid binding; histone binding; chromatin binding

Biological Process: positive regulation of catalytic activity; chromatin remodeling; embryonic development; positive regulation of meiosis; single fertilization; regulation of exit from mitosis; oocyte differentiation; protein homooligomerization; positive regulation of DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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