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NFKBIA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6165594

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFKBIA, Monoclonal Antibody
Full Product Name
NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha, IKBA, MAD-3, NFKBI) (AP)
Product Gene Name
Product Synonym Names
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor; alpha; IKBA; MAD-3; NFKBI
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3H4
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes NFKBIA.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
NFKBIA (AAH04983, 1aa-317aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Conjugate
AP
Applications Tested/Suitable for anti-NFKBIA antibody
Western Blot (WB)
Application Notes for anti-NFKBIA antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFKBIA antibody

NFKBIA monoclonal antibody (M10), clone 3H4. Western Blot analysis of NFKBIA expression in Hela S3 NE.
Mouse Anti-NFKBIA Monoclonal Antibody – Cat# MBS6165594

Testing Data of anti-NFKBIA antibody

Detection limit for recombinant GST tagged NFKBIA is 0.03 ng/ml as a capture antibody.
Mouse Anti-NFKBIA Monoclonal Antibody, Testing Data – Cat# MBS6165594

Western Blot (WB) of anti-NFKBIA antibody

Western Blot analysis of NFKBIA expression in transfected 293T cell line by NFKBIA monoclonal antibody (M10), clone 3H4.

Lane 1: NFKBIA transfected lysate (Predicted MW: 35.6 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-NFKBIA Monoclonal Antibody, Western Blot (WB) – Cat# MBS6165594

Immunofluorescence (IF) of anti-NFKBIA antibody

Immunofluorescence of monoclonal antibody to NFKBIA on HeLa cell. [antibody concentration 20 ug/ml]
Mouse Anti-NFKBIA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6165594

Immunofluorescence (IF) of anti-NFKBIA antibody

Immunofluorescence of monoclonal antibody to NFKBIA on HeLa cell. [antibody concentration 20 ug/ml]
Mouse Anti-NFKBIA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6165594

Other Notes

Small volumes of anti-NFKBIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFKBIA purchase

MBS6165594 is an antibody targeting NFKBIA. Also known as NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha, IKBA, MAD-3, NFKBI) (AP). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFKBIA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
NCBI Official Synonym Full Names
NFKB inhibitor alpha
NCBI Official Symbol
NFKBIA
NCBI Official Synonym Symbols
IKBA; MAD-3; NFKBI; EDAID2
Protein Family
NCBI Protein Information
NF-kappa-B inhibitor alpha

NCBI Summary for NFKBIA

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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