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OXSR1 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616581

SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible… More details

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Product Overview

Online Datasheet in PDF
Product Name
OSR1 (OXSR1), Antibody
Full Product Name
OSR1 (Oxidative Stress Responsive 1)
Product Gene Name
anti-OXSR1 antibody
Product Synonym Names
Anti -OSR1 (Oxidative Stress Responsive 1)
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of total human OSR1 at ~60kD. Does not crossreact with SPAK or other members of the GCK family.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
chromosome: 3; Location: 3p22.2
OMIM
604046
3D Structure
ModBase 3D Structure for O95747
Type
Pab
Grade
Affinity Purified
Immunogen
Synthetic peptide corresponding to residues at the amino terminus of human OSR1
Cross Reactivity
Bovine and Monkey

Product Categories/Family for anti-OXSR1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-OXSR1 antibody
Western Blot (WB), Flow Cytometry (FC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-OXSR1 antibody
Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Flow Cytometry: 1:100
Immunofluorescence (IC): 1:25
Optimal dilutions to be determined by the researcher.
Other applications not tested.

Western Blot (WB) of anti-OXSR1 antibody

Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using MBS616581.
Rabbit Anti-OXSR1 Antibody – Cat# MBS616581

Flow Cytometry (FC/FACS) of anti-OXSR1 antibody

Flow cytometric analysis of HeLa cells using MBS616581 (blue) compared to a nonspecific negative control antibody (red).
Rabbit Anti-OXSR1 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS616581

Immunofluorescence (IF) of anti-OXSR1 antibody

Confocal immunofluorescent analysis of HeLa cells using MBS616581 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Rabbit Anti-OXSR1 Antibody, Immunofluorescence (IF) – Cat# MBS616581

Other Notes

Small volumes of anti-OXSR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OXSR1 purchase

MBS616581 is an antibody targeting OXSR1. Also known as OSR1 (Oxidative Stress Responsive 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for OXSR1. It may not necessarily be applicable to this product.

NCBI GI #
4519629
NCBI GeneID
9943
UniProt Primary Accession #
O95747
UniProt Secondary Accession #
Q3LR53; Q7Z501; Q9UPQ1
UniProt Related Accession #
O95747
NCBI Official Full Name
oxidative-stress responsive 1
NCBI Official Synonym Full Names
oxidative-stress responsive 1
NCBI Official Symbol
OXSR1
NCBI Official Synonym Symbols
OSR1; KIAA1101; OXSR1
UniProt Protein Name
Serine/threonine-protein kinase OSR1
UniProt Synonym Protein Names
Oxidative stress-responsive 1 protein
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
OXSR1
UniProt Synonym Gene Names
KIAA1101; OSR1
UniProt Entry Name
OXSR1_HUMAN
NCBI Protein Information
serine/threonine-protein kinase OSR1; OTTHUMP00000209062; oxidative stress-responsive 1 protein

NCBI Summary for OXSR1

The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq]

UniProt Comments for OXSR1

OSR1: oxidative-stress responsive 1 protein is a ubiquitously expressed protein kinase of the STE20 family. Regulates Na-K-Cl cotransporter (NKCC) activity. Regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton. Binds to and phosphorylates PAK1. Interacts and is phosphorylated by WNK1. Knockdown of either WNK1 or OXSR1 reduces NKCC activity. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress. An important paralog of this gene is STRADB.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; FRAY subfamily

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-threonine phosphorylation; response to oxidative stress; protein amino acid phosphorylation
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Research Articles on OXSR1

1. crystal structure of OSR1 kinase domain has been solved at 2.25 A; OSR1 forms a domain-swapped dimer in an inactive conformation, in which P+1 loop and alphaEF helix are swapped between dimer-related monomers
2. The WNK1-SPAK/OSR1 signalling pathway plays a key role in controlling the phosphorylation and activity of NCC.
3. The first crystal structure of an OSR1 fragment encompassing the catalytic domain of the enzyme, is reported.
4. These results provide the first molecular insight into the mechanism by which the SPAK and OSR1 kinases specifically recognize their upstream activators and downstream substrates.
5. OXSR1 kinase is shown to interact with the three RELT family members RELT, RELL1, and RELL2 by in vitro co-immunoprecipitation; additionally, OXSR1 phosphorylates RELT family members in an in vitro kinase assay.
6. isolation and characterization of OSR1 (oxidative stress-responsive 1), one of two human Fray homologs. OSR1 is a 58-kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines
7. WNK1 and SPAK/OSR1 mediate the hypotonic stress signaling pathway to cation-chloride-coupled cotransporters
8. data establish that the CCT domain functions as a multipurpose docking site, enabling SPAK/OSR1 to interact with substrates (NKCC1) and activators (WNK1/WNK4)
9. OSR1 and sterile20-related, proline-, alanine-rich kinase are likely links between WNK lysine deficient protein kinase 1 and solute carrier family 12 in a pathway that contributes to volume regulation and blood pressure homeostasis in mammals
10. interacts with cation chloride cotransporters
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OXSR1 antibody

 Reference Product  PubMed Publications
 PAK1 antibody  >1 publications with OXSR1 and PAK1

Diseases associated with anti-OXSR1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with OXSR1 and Neoplasms
 Esophageal Neoplasms Antibodies  >1 publications with OXSR1 and Esophageal Neoplasms
 Myocardial Infarction Antibodies  >1 publications with OXSR1 and Myocardial Infarction
 Lung Neoplasms Antibodies  >1 publications with OXSR1 and Lung Neoplasms
 Necrosis Antibodies  >1 publications with OXSR1 and Necrosis

Organs/Tissues associated with anti-OXSR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with OXSR1 and Brain
 Blood Antibodies  >3 publications with OXSR1 and Blood
 Nerve Antibodies  >2 publications with OXSR1 and Nerve
 Prostate Antibodies  >2 publications with OXSR1 and Prostate
 Heart Antibodies  >1 publications with OXSR1 and Heart
 Muscle Antibodies  >1 publications with OXSR1 and Muscle
 Intestine Antibodies  >1 publications with OXSR1 and Intestine
 Pancreas Antibodies  >1 publications with OXSR1 and Pancreas
 Lung Antibodies  >1 publications with OXSR1 and Lung
 Testis Antibodies  >1 publications with OXSR1 and Testis
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