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BACE1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6165850

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this… More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BACE1, Monoclonal Antibody
Full Product Name
BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (AP)
Product Gene Name
Product Synonym Names
beta-site APP-cleaving Enzyme 1; ASP2; BACE; FLJ90568; HSPC104; KIAA1149
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2C1
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified
Specificity
Recognizes BACE1.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
BACE1 (AAH65492, 22aa-501aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK
Conjugate
AP

Product Categories/Family for anti-BACE1 antibody

Applications Tested/Suitable for anti-BACE1 antibody
Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell)
Application Notes for anti-BACE1 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-BACE1 antibody

Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Mouse Anti-BACE1 Monoclonal Antibody – Cat# MBS6165850

Immunohistochemistry (IHC) of anti-BACE1 antibody

Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Mouse Anti-BACE1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6165850

Testing Data of anti-BACE1 antibody

Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.
Mouse Anti-BACE1 Monoclonal Antibody, Testing Data – Cat# MBS6165850

Testing Data of anti-BACE1 antibody

Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-BACE1 Monoclonal Antibody, Testing Data – Cat# MBS6165850

Western Blot (WB) of anti-BACE1 antibody

Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.

Lane 1: BACE1 transfected lysate(52.9 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-BACE1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6165850

Western Blot (WB) of anti-BACE1 antibody

BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.
Mouse Anti-BACE1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6165850

Other Notes

Small volumes of anti-BACE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BACE1 purchase

MBS6165850 is an antibody targeting BACE1. Also known as BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (AP). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for BACE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Beta-site APP-cleaving enzyme 1
NCBI Official Synonym Full Names
beta-secretase 1
NCBI Official Symbol
BACE1
NCBI Official Synonym Symbols
ASP2; BACE; HSPC104
Protein Family
NCBI Protein Information
beta-secretase 1

NCBI Summary for BACE1

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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