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TRIB3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6166565

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TRIB3, Monoclonal Antibody
Full Product Name
TRIB3 (Tribbles Homolog 3 (Drosophila), C20orf97, NIPK, SINK, SKIP3, TRB3) (APC)
Product Gene Name
Product Synonym Names
Tribbles Homolog 3 (Drosophila); C20orf97; NIPK; SINK; SKIP3; TRB3
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3D7
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes TRIB3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
TRIB3 (AAH27484, 56aa-145aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
PDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMH
Conjugate
APC

Product Categories/Family for anti-TRIB3 antibody

Applications Tested/Suitable for anti-TRIB3 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TRIB3 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-TRIB3 antibody

Detection limit for recombinant GST tagged TRIB3 is 3 ng/ml as a capture antibody.
Mouse Anti-TRIB3 Monoclonal Antibody – Cat# MBS6166565

Western Blot (WB) of anti-TRIB3 antibody

Western Blot analysis of TRIB3 expression in transfected 293T cell line by TRIB3 monoclonal antibody (M07), clone 3D7.

Lane 1: TRIB3 transfected lysate(39.6 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-TRIB3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6166565

Immunoprecipitation (IP) of anti-TRIB3 antibody

Immunoprecipitation of TRIB3 transfected lysate using anti-TRIB3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TRIB3 MaxPab rabbit polyclonal antibody.
Mouse Anti-TRIB3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6166565

Other Notes

Small volumes of anti-TRIB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIB3 purchase

MBS6166565 is an antibody targeting TRIB3. Also known as TRIB3 (Tribbles Homolog 3 (Drosophila), C20orf97, NIPK, SINK, SKIP3, TRB3) (APC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIB3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Tribbles homolog 3 (Drosophila)
NCBI Official Synonym Full Names
tribbles pseudokinase 3
NCBI Official Symbol
TRIB3
NCBI Official Synonym Symbols
NIPK; SINK; TRB3; SKIP3; C20orf97
Protein Family
NCBI Protein Information
tribbles homolog 3

NCBI Summary for TRIB3

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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