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PKP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6166804

Armadillo-like proteins are characterized by a series of armadillo repeats, first defined in the Drosophila 'armadillo' gene product, that are typically 42 to 45 amino acids in length. These proteins can be divided into subfamilies based on their number of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PKP, Monoclonal Antibody
Full Product Name
PKP (Plakophilin 4, FLJ31261, FLJ42243, p0071) (APC)
Product Gene Name
Product Synonym Names
Plakophilin 4; FLJ31261; FLJ42243; p0071
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4H7
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes PKP4.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
127kDa
OMIM
604276
3D Structure
ModBase 3D Structure for Q99569
Immunogen
PKP4 (NP_001005476, 12aa-110aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EGQPQTRQEAASTGPGMEPETTATTILASVKEQELQFQRLTRELEVERQIVASQLERCRLGAESPSIASTSSTEKSFPWRSTDVPNTGVSKPRVSDAVQ
Conjugate
APC

Product Categories/Family for anti-PKP antibody

Applications Tested/Suitable for anti-PKP antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PKP antibody
Applications are based on unconjugated antibody.

Testing Data of anti-PKP antibody

Detection limit for recombinant GST tagged PKP4 is approximately 10ng/ml as a capture antibody.
Mouse Anti-PKP Monoclonal Antibody – Cat# MBS6166804

Immunofluorescence (IF) of anti-PKP antibody

Immunofluorescence of monoclonal antibody to PKP4 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PKP Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166804

Immunofluorescence (IF) of anti-PKP antibody

Immunofluorescence of monoclonal antibody to PKP4 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PKP Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166804

Other Notes

Small volumes of anti-PKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKP purchase

MBS6166804 is an antibody targeting PKP. Also known as PKP (Plakophilin 4, FLJ31261, FLJ42243, p0071) (APC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PKP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
plakophilin-4 isoform b
NCBI Official Synonym Full Names
plakophilin 4
NCBI Official Symbol
PKP4
NCBI Official Synonym Symbols
p0071
UniProt Protein Name
Plakophilin-4
UniProt Synonym Protein Names
p0071
UniProt Gene Name
PKP4
UniProt Entry Name
PKP4_HUMAN
NCBI Protein Information
plakophilin-4

NCBI Summary for PKP

Armadillo-like proteins are characterized by a series of armadillo repeats, first defined in the Drosophila 'armadillo' gene product, that are typically 42 to 45 amino acids in length. These proteins can be divided into subfamilies based on their number of repeats, their overall sequence similarity, and the dispersion of the repeats throughout their sequences. Members of the p120(ctn)/plakophilin subfamily of Armadillo-like proteins, including CTNND1, CTNND2, PKP1, PKP2, PKP4, and ARVCF. PKP4 may be a component of desmosomal plaque and other adhesion plaques and is thought to be involved in regulating junctional plaque organization and cadherin function. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]
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UniProt Comments for PKP

plakophilin 4: an armadillo-like protein of the beta-catenin family. A component of desmosomal plaques and other adhesion plaques and is thought to be involved in regulating junctional plaque organization and cadherin function. Two splice-variant isoforms have been described

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 2q24.1

Cellular Component: spindle pole; desmosome; internal side of plasma membrane; cytoskeleton; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; plasma membrane; midbody; intercellular junction; spindle midzone

Molecular Function: protein binding

Biological Process: positive regulation of cytokinesis; regulation of cell adhesion; intercellular junction assembly; cell-cell adhesion; cell-cell signaling
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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