Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1) Antibody – Goat Polyclonal

Suitable for use in Western blot, Immunohistochemistry, and Direct ELISA., , Recommended Dilutions:, Western Blot: 0.1-0.2ug/ml. The detection limit is ~2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively., Immunohistochemistry: 10ug/ml detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4 degree C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at RT. After, blocking, sections were incubated with primary antibody for 3 hours at RT followed by NorthernLightsTM 557 donkey anti-goat lgG at RT for 1 hour. Between each step, sections were, washed with 0.1% BSA in PBS., Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.5 ng/well., Optimal dilutions to be determined by the researcher., , Storage and Stability:, Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.