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NFKB1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6167283

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
NFKB1, Monoclonal Antibody
Full Product Name
NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (APC)
Product Gene Name
anti-NFKB1 antibody
Product Synonym Names
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1; DKFZp686C01211; EBP-1; KBF1; MGC54151; NF-kappa-B; NFKB-p105; NFKB-p50; p105; p50
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4A11
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes NFKB1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
NFKB1 (AAH51765, 860aa-969aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
Conjugate
APC

Product Categories/Family for anti-NFKB1 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-NFKB1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell)
Application Notes for anti-NFKB1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFKB1 antibody

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Lane 1: NFKB1 transfected lysate(105.4 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-NFKB1 Monoclonal Antibody – Cat# MBS6167283

Immunohistochemistry (IHC) of anti-NFKB1 antibody

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Mouse Anti-NFKB1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6167283

Immunohistochemistry (IHC) of anti-NFKB1 antibody

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Mouse Anti-NFKB1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6167283

Testing Data of anti-NFKB1 antibody

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.
Mouse Anti-NFKB1 Monoclonal Antibody, Testing Data – Cat# MBS6167283

Testing Data of anti-NFKB1 antibody

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-NFKB1 Monoclonal Antibody, Testing Data – Cat# MBS6167283

Immunofluorescence (IF) of anti-NFKB1 antibody

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-NFKB1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6167283

Other Notes

Small volumes of anti-NFKB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFKB1 purchase

MBS6167283 is an antibody targeting NFKB1. Also known as NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (APC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NFKB1. It may not necessarily be applicable to this product.

NCBI GI #
30354570
NCBI GeneID
4790
NCBI Accession #
AAH51765
UniProt Related Accession #
P19838
NCBI Official Full Name
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
NCBI Official Synonym Full Names
nuclear factor kappa B subunit 1
NCBI Official Symbol
NFKB1
NCBI Official Synonym Symbols
p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1
Protein Family
Nuclear factor
NCBI Protein Information
nuclear factor NF-kappa-B p105 subunit

NCBI Summary for NFKB1

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
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Research Articles on NFKB1

1. NF-kappaB expression and signaling is activated by Candida albicans in the oral lichen planus-derived keratinocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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