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PUF60 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6169290

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PUF60, Monoclonal Antibody
Full Product Name
PUF60 (poly-U Binding Splicing Factor 60kD, FIR, FLJ31379, RoBPI, SIAHBP1) (APC)
Product Gene Name
Product Synonym Names
poly-U Binding Splicing Factor 60kD; FIR; FLJ31379; RoBPI; SIAHBP1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1C1
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes PUF60.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
PUF60 (AAH11979, 1aa-516aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Conjugate
APC

Product Categories/Family for anti-PUF60 antibody

Applications Tested/Suitable for anti-PUF60 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PUF60 antibody
Applications are based on unconjugated antibody.

Testing Data of anti-PUF60 antibody

Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.
Mouse Anti-PUF60 Monoclonal Antibody – Cat# MBS6169290

Western Blot (WB) of anti-PUF60 antibody

Western Blot analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1.

Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-PUF60 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6169290

Immunofluorescence (IF) of anti-PUF60 antibody

Immunofluorescence of monoclonal antibody to PUF60 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PUF60 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6169290

Immunofluorescence (IF) of anti-PUF60 antibody

Immunofluorescence of monoclonal antibody to PUF60 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PUF60 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6169290

Other Notes

Small volumes of anti-PUF60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUF60 purchase

MBS6169290 is an antibody targeting PUF60. Also known as PUF60 (poly-U Binding Splicing Factor 60kD, FIR, FLJ31379, RoBPI, SIAHBP1) (APC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
PUF60 protein
NCBI Official Synonym Full Names
poly(U) binding splicing factor 60
NCBI Official Symbol
PUF60
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
NCBI Protein Information
poly(U)-binding-splicing factor PUF60

NCBI Summary for PUF60

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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