Alpha (HE2) Antibody – Rabbit Polyclonal

HE2 is a human epididymis specific sperm binding secretory protein expressed by SPAG11 gene (chromosome 8p23-p22), a 103aa protein adopts a typical antigenic pattern with a subacrosomal equatorial distribution on the sperm head, this localization of HE2 with Beta-defensins in the epithelial defense system of epididymal duct have developed mechanism to fight ascending infections, including local production of these antimicrobial peptides and proteins. The regionalized epididymal expression of the HE2 and its isoforms suggests that they exert their effects at different times in the controlled sequence of sperm modification, maturation, binding and a direct role in fertilization. HE2 represents 3 major isoforms, HE2-alpha, HE2-beta and HE2-gamma, all variants have a common 71 residue N terminus but various C termini, these variations at C termini is due to frame shifting deletions. Thus, identical sequences and deletion, start and stop points indicate that HE2 isoforms are derived from alternative splicing of 8 or more exons of a single gene and working towards common microbial defense and sperm maturation to acquire fertilizing ability. The human HE2 protein and its isoforms are 28% conserved. A gene product of rat epididymal duct, BIN1B, has been suggested to represent the rat counterpart of human HE2 protein. As HE2, BIN1B also shows structural characteristics of beta-defensins. HE2 alpha: It appears in two isoforms, HE2 A1 (103aa) and HE2 A2 (70aa). HE2 alpha represents major peptide isoforms in human epididymis, processed at the peptide level and secreted apically into the duct lumen, HE2 A2 sequence is entirely different from defensins and from any other known peptide. Epididymis restricted expression and sperm binding properties of HE2 alpha suggests its involvement in the process of post-testicular sperm maturation.

• 1. Hans Henning von Horsten et al (2002) Biol of reproduction 67, 804-813; Hamil, K.G et al (2000) Endocrinology 141 (3), 1245-1253;  Peng Li et al (2001) Science 291, 1783-1785; Katherine G Hamil et al (2000) Endocrinology 141, 1245-1253.

Small volumes of anti-HE2, alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS617002 is an antibody targeting HE2, alpha. Also known as Anti - HE2, alpha. Reactive in Human. Host: Rabbit
Source: Human.