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ZNF274 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6170692

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZNF274, Monoclonal Antibody
Full Product Name
ZNF274 (Zinc Finger Protein 274, DKFZp686K08243, FLJ37843, HFB101, ZF2, ZKSCAN19) (Biotin)
Product Gene Name
Product Synonym Names
Zinc Finger Protein 274; DKFZp686K08243; FLJ37843; HFB101; ZF2; ZKSCAN19
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1D8
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified
Specificity
Recognizes ZNF274.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
605467
3D Structure
ModBase 3D Structure for Q96GC6
Immunogen
ZNF274 (NP_598009, 420aa-530aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT
Conjugate
Biotin
Applications Tested/Suitable for anti-ZNF274 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-ZNF274 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ZNF274 antibody

Western blot analysis of ZNF274 over-expressed 293 cell line, cotransfected with ZNF274 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF274 monoclonal antibody (M04), clone 1D8. GAPDH (36.1 kDa) used as specificity and loading control.
Mouse Anti-ZNF274 Monoclonal Antibody – Cat# MBS6170692

Western Blot (WB) of anti-ZNF274 antibody

Western Blot analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M04), clone 1D8.

Lane 1: ZNF274 transfected lysate(74.2 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-ZNF274 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6170692

Immunofluorescence (IF) of anti-ZNF274 antibody

Immunofluorescence of monoclonal antibody to ZNF274 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-ZNF274 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6170692

Immunofluorescence (IF) of anti-ZNF274 antibody

Immunofluorescence of monoclonal antibody to ZNF274 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-ZNF274 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6170692

Other Notes

Small volumes of anti-ZNF274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZNF274 purchase

MBS6170692 is an antibody targeting ZNF274. Also known as ZNF274 (Zinc Finger Protein 274, DKFZp686K08243, FLJ37843, HFB101, ZF2, ZKSCAN19) (Biotin). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ZNF274. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neurotrophin receptor-interacting factor homolog isoform c
NCBI Official Synonym Full Names
zinc finger protein 274
NCBI Official Symbol
ZNF274
NCBI Official Synonym Symbols
ZF2; HFB101; ZSCAN51; ZKSCAN19
UniProt Protein Name
Neurotrophin receptor-interacting factor homolog
UniProt Synonym Protein Names
Zinc finger protein 274; Zinc finger protein HFB101; Zinc finger protein with KRAB and SCAN domains 19; Zinc finger protein zfp2; Zf2
UniProt Gene Name
ZNF274
UniProt Synonym Gene Names
ZKSCAN19; SP2114; Zf2
UniProt Entry Name
ZN274_HUMAN
NCBI Protein Information
neurotrophin receptor-interacting factor homolog

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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