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PBK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6171340

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PBK, Monoclonal Antibody
Full Product Name
PBK (PDZ Binding Kinase, FLJ14385, Nori-3, SPK, TOPK) (Biotin)
Product Gene Name
Product Synonym Names
PDZ Binding Kinase; FLJ14385; Nori-3; SPK; TOPK
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3A7
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified
Specificity
Recognizes PBK.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PBK (AAH15191, 1aa-110aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGS
Conjugate
Biotin

Product Categories/Family for anti-PBK antibody

Applications Tested/Suitable for anti-PBK antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PBK antibody
Applications are based on unconjugated antibody.

Testing Data of anti-PBK antibody

Immunoperoxidase of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Mouse Anti-PBK Monoclonal Antibody – Cat# MBS6171340

Immunohistochemistry (IHC) of anti-PBK antibody

Immunoperoxidase of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Mouse Anti-PBK Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6171340

Immunofluorescence (IF) of anti-PBK antibody

Immunofluorescence of monoclonal antibody to PBK on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PBK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6171340

Immunofluorescence (IF) of anti-PBK antibody

Immunofluorescence of monoclonal antibody to PBK on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-PBK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6171340

Western Blot (WB) of anti-PBK antibody

PBK monoclonal antibody (M07), clone 3A7 Western Blot analysis of PBK expression in Hela S3 NE.
Mouse Anti-PBK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6171340

Western Blot (WB) of anti-PBK antibody

PBK monoclonal antibody (M07), clone 3A7. Western Blot analysis of PBK expression in 293.
Mouse Anti-PBK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6171340

Other Notes

Small volumes of anti-PBK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PBK purchase

MBS6171340 is an antibody targeting PBK. Also known as PBK (PDZ Binding Kinase, FLJ14385, Nori-3, SPK, TOPK) (Biotin). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PBK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
PDZ binding kinase
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
PBK
NCBI Official Synonym Symbols
SPK; CT84; TOPK; HEL164; Nori-3
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase

NCBI Summary for PBK

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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