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AKT2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6172077

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AKT2, Monoclonal Antibody
Full Product Name
AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (Biotin)
Product Gene Name
Product Synonym Names
v-akt Murine Thymoma Viral Oncogene Homolog 2; PKBB; PKBBETA; PRKBB; RAC-BETA
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1D9
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified
Specificity
Recognizes AKT2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
AKT2 (AAA58364, 100aa-189aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVII
Conjugate
Biotin

Product Categories/Family for anti-AKT2 antibody

Applications Tested/Suitable for anti-AKT2 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-AKT2 antibody
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-AKT2 antibody

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-AKT2 Monoclonal Antibody – Cat# MBS6172077

Immunofluorescence (IF) of anti-AKT2 antibody

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-AKT2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6172077

Western Blot (WB) of anti-AKT2 antibody

AKT2 monoclonal antibody (M03), clone 1D9 Western Blot analysis of AKT2 expression in Jurkat.
Mouse Anti-AKT2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6172077

Testing Data of anti-AKT2 antibody

Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.
Mouse Anti-AKT2 Monoclonal Antibody, Testing Data – Cat# MBS6172077

Western Blot (WB) of anti-AKT2 antibody

AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in NIH/3T3.
Mouse Anti-AKT2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6172077

Western Blot (WB) of anti-AKT2 antibody

AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in PC-12.
Mouse Anti-AKT2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6172077

Product References and Citations for anti-AKT2 antibody

1. Multiple defects in negative regulation of the PKB/Akt pathway sensitise human cancer cells to the antiproliferative effect of non-steroidal anti-inflammatory drugs. Lincova E, Hampl A, Pernicova Z, Star?ichova A, Kr?ma? P, Machala M, Kozubik A, Sou?ek K.Biochem Pharmacol. 2009 Sep 15;78(6):561-72. Epub 2009 May 9.

Other Notes

Small volumes of anti-AKT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKT2 purchase

MBS6172077 is an antibody targeting AKT2. Also known as AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (Biotin). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
protein serine/threonine kinase
NCBI Official Synonym Full Names
AKT serine/threonine kinase 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Protein Family
NCBI Protein Information
RAC-beta serine/threonine-protein kinase

NCBI Summary for AKT2

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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