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HNF4A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6172502

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HNF4A, Monoclonal Antibody
Full Product Name
HNF4A (Hepatocyte Nuclear Factor 4, alpha, FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14) (Biotin)
Product Gene Name
Product Synonym Names
Hepatocyte Nuclear Factor 4; alpha; FLJ39654; HNF4; HNF4a7; HNF4a8; HNF4a9; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4D9
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified
Specificity
Recognizes HNF4A.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
43,988 Da
OMIM
125850
3D Structure
ModBase 3D Structure for A5JW41
Immunogen
HNF4A (NP_000448, 324aa-423aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
NDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRP
Conjugate
Biotin
Applications Tested/Suitable for anti-HNF4A antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-HNF4A antibody
Applications are based on unconjugated antibody.

Testing Data of anti-HNF4A antibody

Detection limit for recombinant GST tagged HNF4A is 0.1 ng/ml as a capture antibody.
Mouse Anti-HNF4A Monoclonal Antibody – Cat# MBS6172502

Western Blot (WB) of anti-HNF4A antibody

Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M06), clone 4D9.

Lane 1: HNF4A transfected lysate (Predicted MW: 51.6 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-HNF4A Monoclonal Antibody, Western Blot (WB) – Cat# MBS6172502

Immunofluorescence (IF) of anti-HNF4A antibody

Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-HNF4A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6172502

Immunofluorescence (IF) of anti-HNF4A antibody

Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-HNF4A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6172502

Other Notes

Small volumes of anti-HNF4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNF4A purchase

MBS6172502 is an antibody targeting HNF4A. Also known as HNF4A (Hepatocyte Nuclear Factor 4, alpha, FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14) (Biotin). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HNF4A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
NCBI Official Synonym Full Names
hepatocyte nuclear factor 4, alpha
NCBI Official Symbol
HNF4A
NCBI Official Synonym Symbols
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
UniProt Protein Name
Hepatocyte nuclear factor 4-alpha
UniProt Synonym Protein Names
Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
UniProt Gene Name
HNF4A
UniProt Synonym Gene Names
HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
UniProt Entry Name
HNF4A_HUMAN
NCBI Protein Information
hepatocyte nuclear factor 4-alpha; HNF4alpha10/11/12; TCF-14; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1; transcription factor 14; transcription factor HNF-4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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