Rabbit Anti-CREB1 Polyclonal Antibody – Cat# MBS617309

Western blot of whole cell lysate from SK-N-MC cells using MBS617309, untreated (-) or treated with 3 μM forskolin

CREB, Ser133 (CREB1) Antibody – Rabbit Polyclonal

Name: CREB1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS617309
Price: 1015 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617309

By immunoblotting the antibody shows a 5-10 fold increase in staining of a 43kD protein after treatment of cells with agents that increase intracellular cAMP. The antibody also recognizes phosphorylated ATF-1 due to a common epitope. The antibody has been… More details

$1015 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CREB, Ser133 (CREB1), Polyclonal Antibody

Full Product Name

CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)

Product Gene Name

anti-CREB1 antibody

Product Synonym Names

Anti -CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Monkey, Mouse, Rat

Form/Format

Supplied as a liquid with 0.01% sodium azide.

Purity/Purification

Serum
Cross adsorbed serum

Specificity

Recognizes phosphorylated Serine133 in cAMP response element binding protein. (CREB). Species Crossreactivity: Human, rat, monkey and mouse.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

34,877 Da

Chromosome Location

Chromosome: 2; NC_007300.4 (100928794..100961320)

3D Structure

ModBase 3D Structure for P27925

Immunogen

Phosphopeptide conjugated to KLH.

Product Categories/Family for anti-CREB1 antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-CREB1 antibody

Western Blot (WB)

Application Notes for anti-CREB1 antibody

Suitable for use in Immunoblot.
Dilution: Immunoblot: 1:500-1:1000
Optimal dilution determined by the researcher.

Other Notes

Small volumes of anti-CREB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CREB1 purchase

MBS617309 is an antibody targeting CREB1. Also known as CREB, phosphorylated, Ser133 (cAMP Response Element Binding Protein). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CREB1. It may not necessarily be applicable to this product.

NCBI GI #

27805857

NCBI GeneID

281713

NCBI Accession #

NP_776710.1

UniProt Primary Accession #

P27925

UniProt Secondary Accession #

O18957; A5PK02

UniProt Related Accession #

P27925; Q0V7N6

GenBank Nucleotide #

NM_174285.1

NCBI Official Full Name

cyclic AMP-responsive element-binding protein 1

NCBI Official Symbol

CREB1

NCBI Official Synonym Symbols

CREB

UniProt Protein Name

Cyclic AMP-responsive element-binding protein 1

UniProt Synonym Protein Names

Cyclic AMP-responsive DNA-binding protein

Protein Family

Cyclic AMP-responsive element-binding protein

UniProt Gene Name

CREB1

UniProt Synonym Gene Names

CREB; CREB2

UniProt Entry Name

CREB1_BOVIN

NCBI Protein Information

cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; cyclic AMP-responsive DNA-binding protein

UniProt Comments for CREB1

Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Implicated in synchronization of circadian rhythmicity

By similarity. Ref.4 Ref.5 Ref.6

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-117, binds CREBBP. Interacts (phosporylated form) with TOX3

Subcellular location: Nucleus.

Post-translational modification: Sumoylated by SUMO1. Sumoylation on Lys-288, but not on Lys-269, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

By similarity.Stimulated by phosphorylation. Phosphorylation of both Ser-117 and Ser-126 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-117 allows CREBBP binding. Phosphorylated upon DNA damage, probably by ATM or ATR

By similarity. Ref.7

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain.

Research Articles on CREB1

1. dsRNA as well as other agonists of endothelial cells elicit signaling mechanisms that include in part CREB phosphorylation mediated by calcium-independent phospholipase A2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CREB1 antibody

Products by Pathway	Pathway Diagram
AKT Phosphorylates Targets In The Nucleus Pathway antibodies	AKT Phosphorylates Targets In The Nucleus Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Antigen Processing And Presentation Pathway antibodies	Antigen Processing And Presentation Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
CREB Phosphorylation Pathway antibodies	CREB Phosphorylation Pathway Diagram
Ca-dependent Events Pathway antibodies	Ca-dependent Events Pathway Diagram
CaM Pathway antibodies	CaM Pathway Diagram
CaMK IV-mediated Phosphorylation Of CREB Pathway antibodies	CaMK IV-mediated Phosphorylation Of CREB Pathway Diagram

Diseases associated with anti-CREB1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>109 publications with CREB1 and Neoplasms
Mental Disorders Antibodies	>54 publications with CREB1 and Mental Disorders
Skin Diseases Antibodies	>31 publications with CREB1 and Skin Diseases
Inflammation Antibodies	>31 publications with CREB1 and Inflammation
Leukemia Antibodies	>30 publications with CREB1 and Leukemia
Depressive Disorder Antibodies	>26 publications with CREB1 and Depressive Disorder
Necrosis Antibodies	>25 publications with CREB1 and Necrosis
Carcinoma Antibodies	>22 publications with CREB1 and Carcinoma
Breast Neoplasms Antibodies	>19 publications with CREB1 and Breast Neoplasms
Cardiovascular Diseases Antibodies	>18 publications with CREB1 and Cardiovascular Diseases

Organs/Tissues associated with anti-CREB1 antibody

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>8 publications with CREB1 and Pancreas
Ovary Antibodies	>7 publications with CREB1 and Ovary
Intestine Antibodies	>6 publications with CREB1 and Intestine