Goat Anti-NFIL3 Polyclonal Antibody – Cat# MBS617327

NFIL3 Antibody – Goat Polyclonal

Name: NFIL3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS617327
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS617327

A basic leucine zipper transcription factor, which activates IL3 gene expression and can also repress transcription. More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NFIL3, Polyclonal Antibody

Full Product Name

NFIL3 (E4 Promoter Binding Protein 4, E4BP4, E4BP4 Protein, Interleukin 3 Binding Protein 1, IL3BP1, Interleukin 3 Promoter Transcriptional Activator, NFIL3A, Nuclear Factor Interleukin 3 Regulated, Transcriptional Activator NF-IL3A)

Product Gene Name

anti-NFIL3 antibody

Product Synonym Names

Anti -NFIL3 (E4 Promoter Binding Protein 4, E4BP4, E4BP4 Protein, Interleukin 3 Binding Protein 1, IL3BP1, Interleukin 3 Promoter Transcriptional Activator, NFIL3A, Nuclear Factor Interleukin 3 Regulated, Transcriptional Activator NF-IL3A)

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human NFIL3. Species Crossreactivity: Human. Expected to crossreact with Rat and Mouse due to sequence homology.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

51,472 Da

Chromosome Location

Chromosome: 9; NC_000009.11 (94171327..94186144, complement). Location: 9q22

OMIM

605327

3D Structure

ModBase 3D Structure for Q16649

Immunogen

Synthetic peptide with sequence RLIATQPISASDSG, representing the C-Terminus of the protein (residues 449-462) according to NP_005375.

Product Categories/Family for anti-NFIL3 antibody

Antibodies; Abs to Transcription Factors

Applications Tested/Suitable for anti-NFIL3 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-NFIL3 antibody

Suitable for use in Western Blot.
Dilution: ELISA: 1:8000
Western Blot: ~0.5-1ug/ml. 5~5-60kD band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kD according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243).

Other Notes

Small volumes of anti-NFIL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFIL3 purchase

MBS617327 is an antibody targeting NFIL3. Also known as NFIL3 (E4 Promoter Binding Protein 4, E4BP4, E4BP4 Protein, Interleukin 3 Binding Protein 1, IL3BP1, Interleukin 3 Promoter Transcriptional Activator, NFIL3A, Nuclear Factor Interleukin 3 Regulated, Transcriptional Activator NF-IL3A). Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for NFIL3. It may not necessarily be applicable to this product.

NCBI GI #

52630429

NCBI GeneID

4783

NCBI Accession #

NP_005375.2

UniProt Primary Accession #

Q16649

UniProt Secondary Accession #

Q14211; Q6FGQ8; Q96HS0; B2R9Y8

UniProt Related Accession #

Q16649

GenBank Nucleotide #

NM_005384.2

NCBI Official Full Name

nuclear factor interleukin-3-regulated protein

NCBI Official Synonym Full Names

nuclear factor, interleukin 3 regulated

NCBI Official Symbol

NFIL3

NCBI Official Synonym Symbols

E4BP4; IL3BP1; NFIL3A; NF-IL3A

UniProt Protein Name

Nuclear factor interleukin-3-regulated protein

UniProt Synonym Protein Names

E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A

Protein Family

Nuclear factor interleukin-3-regulated protein

UniProt Gene Name

NFIL3

UniProt Synonym Gene Names

E4BP4; IL3BP1

UniProt Entry Name

NFIL3_HUMAN

NCBI Protein Information

nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; E4 promoter-binding protein 4; interleukin-3-binding protein 1; transcriptional activator NF-IL3A; nuclear factor interleukin 3 regulated protein; interleukin-3 promoter transcriptional activator

NCBI Summary for NFIL3

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM]

UniProt Comments for NFIL3

E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; immune response; negative regulation of transcription from RNA polymerase II promoter

Research Articles on NFIL3

1. Conclude that E4BP4 plays as a survival factor in heart and E4BP4 is essential for proper embryonic heart development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NFIL3 antibody

Reference Product	PubMed Publications
DR1 antibody	>1 publications with NFIL3 and DR1

Diseases associated with anti-NFIL3 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>12 publications with NFIL3 and Neoplasms
Carcinoma Antibodies	>5 publications with NFIL3 and Carcinoma
Adenocarcinoma Antibodies	>4 publications with NFIL3 and Adenocarcinoma
Inflammation Antibodies	>4 publications with NFIL3 and Inflammation
Carcinoma, Hepatocellular Antibodies	>3 publications with NFIL3 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>3 publications with NFIL3 and Liver Diseases
Liver Neoplasms Antibodies	>3 publications with NFIL3 and Liver Neoplasms
Cardiovascular Diseases Antibodies	>2 publications with NFIL3 and Cardiovascular Diseases
Neurodegenerative Diseases Antibodies	>2 publications with NFIL3 and Neurodegenerative Diseases
Female Urogenital Diseases Antibodies	>2 publications with NFIL3 and Female Urogenital Diseases

Organs/Tissues associated with anti-NFIL3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>22 publications with NFIL3 and Blood
Liver Antibodies	>7 publications with NFIL3 and Liver
Muscle Antibodies	>5 publications with NFIL3 and Muscle
Nerve Antibodies	>4 publications with NFIL3 and Nerve
Bone Antibodies	>4 publications with NFIL3 and Bone
Brain Antibodies	>4 publications with NFIL3 and Brain
Parathyroid Antibodies	>3 publications with NFIL3 and Parathyroid
Vascular Antibodies	>2 publications with NFIL3 and Vascular
Heart Antibodies	>2 publications with NFIL3 and Heart
Lung Antibodies	>2 publications with NFIL3 and Lung